Prevalence and characterization of hybrid blaCTX-M among Escherichia coli isolates from livestock and other animals

Pak-Leung Ho, Melissa Chun-Jiao Liu, Wai-U Lo, Eileen Ling-Yi Lai, Terrence Chi-Kong Lau, Oi-Kwan Law, Kin-Hung Chow

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15 Citations (SciVal)

Abstract

This study investigated 248 extended-spectrum β-lactamase-producing Escherichia coli isolates from 2012 to 2013 for hybrid blaCTX-M genes. blaCTX-M genes were detected in 228 isolates of which 14 isolates were hybrid blaCTX-M positive (6 blaCTX-M-123, 6 blaCTX-M-64, and 2 blaCTX-M-132). The 14 hybrid blaCTX-M-carrying isolates (8 from chickens, 2 each from pigs and cattle, 1 each from dog and rodent) were genetically diverse. All but 2 hybrid blaCTX-M were carried on IncI1 (5 blaCTX-M-123) and IncI2 (6 blaCTX-M-64 and one blaCTX-M-132) plasmids. Our IncI1 and IncI2 plasmids had pHNAH4-1-like and pHN1122-1-like restriction fragment length polymorphism patterns, respectively. Genetic relatedness of the plasmids to pHNAH4-1 and pHN1122-1 were confirmed by complete sequencing of 3 plasmids, pCTXM123_C0996, pCTXM64_C0967, and pCTXM132_P0421. Plasmids closely related to pHNAH4-1 and pHN1122-1 and carrying different blaCTX-M alleles have been reported from multiple geographic areas in China previously. The findings highlighted the wide dissemination of hybrid blaCTX-M variants in different parts of China.

Original languageEnglish
Pages (from-to)148-53
Number of pages6
JournalDiagnostic Microbiology and Infectious disease
Volume82
Issue number2
DOIs
Publication statusPublished - Jun 2015

Bibliographical note

Copyright © 2015 Elsevier Inc. All rights reserved.

Keywords

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Animals, Domestic/microbiology
  • China/epidemiology
  • Escherichia coli/enzymology
  • Escherichia coli Infections/microbiology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Plasmids/analysis
  • Prevalence
  • Recombination, Genetic
  • Restriction Mapping
  • Rodentia/microbiology
  • Sequence Analysis, DNA
  • Urinary Tract Infections/epidemiology
  • Young Adult
  • beta-Lactamases/genetics

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