Predicting ligand-free cell attachment on next generation cellulose-chitosan hydrogels

Marcus Johns, Yongho Bae, Francisco Guimarães, Evandro M. Lanzoni, Carlos A. Costa, Paul Murray, Christoph Deneke, Fernando Galembeck, Janet Scott, Ram Sharma

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

There is a growing appreciation that engineered biointerfaces can regulate cell behaviours, or functions. Most systems aim to mimic the cell-friendly extracellular matrix environment, and incorporate protein ligands however, understanding of how a ligand-free system can achieve this is limited. Cell scaffold materials comprised of homogeneous, interfused chitosan-cellulose hydrogels promote cell attachment in ligand-free systems and we demonstrate the role of cellulose molecular weight, MW, and chitosan content and MW in controlling material properties and thus regulating cell attachment. Interpenetrating network gels, generated from cellulose/ionic liquid/co-solvent solutions, using chitosan solutions as phase inversion solvents, were stable and obviated the need for chemical coupling. Interface properties, including surface ζ-potential, dielectric constant, surface roughness, and shear modulus, were modified by varying the chitosan degree of polymerisation and solution concentration, s well as the source of cellulose, creating a family of cellulose-chitosan IPN materials. These features, in turn, affect cell attachment onto the hydrogels and the utility of this ligand-free approach is extended by forecasting cell attachment using regression modelling to isolate the effects of individual parameters in an initially complex system. We demonstrate that increasing the charge density, and/or shear modulus, of the hydrogel results in increased cell attachment.
LanguageEnglish
Pages937–945
JournalACS OMEGA
Volume3
Issue number1
Early online date25 Jan 2018
DOIs
StatusPublished - 31 Jan 2018

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Hydrogels
Chitosan
Cellulose
Ligands
Elastic moduli
Ionic Liquids
Interpenetrating polymer networks
Hydrogel
Zeta potential
Charge density
Ionic liquids
Scaffolds
Surface properties
Large scale systems
Materials properties
Permittivity
Gels
Surface roughness
Molecular weight
Polymerization

Cite this

Johns, M., Bae, Y., Guimarães, F., Lanzoni, E. M., Costa, C. A., Murray, P., ... Sharma, R. (2018). Predicting ligand-free cell attachment on next generation cellulose-chitosan hydrogels. ACS OMEGA, 3(1), 937–945. https://doi.org/10.1021/acsomega.7b01583

Predicting ligand-free cell attachment on next generation cellulose-chitosan hydrogels. / Johns, Marcus; Bae, Yongho; Guimarães, Francisco; Lanzoni, Evandro M.; Costa, Carlos A.; Murray, Paul; Deneke, Christoph; Galembeck, Fernando; Scott, Janet; Sharma, Ram.

In: ACS OMEGA, Vol. 3, No. 1, 31.01.2018, p. 937–945.

Research output: Contribution to journalArticle

Johns, M, Bae, Y, Guimarães, F, Lanzoni, EM, Costa, CA, Murray, P, Deneke, C, Galembeck, F, Scott, J & Sharma, R 2018, 'Predicting ligand-free cell attachment on next generation cellulose-chitosan hydrogels', ACS OMEGA, vol. 3, no. 1, pp. 937–945. https://doi.org/10.1021/acsomega.7b01583
Johns M, Bae Y, Guimarães F, Lanzoni EM, Costa CA, Murray P et al. Predicting ligand-free cell attachment on next generation cellulose-chitosan hydrogels. ACS OMEGA. 2018 Jan 31;3(1):937–945. https://doi.org/10.1021/acsomega.7b01583
Johns, Marcus ; Bae, Yongho ; Guimarães, Francisco ; Lanzoni, Evandro M. ; Costa, Carlos A. ; Murray, Paul ; Deneke, Christoph ; Galembeck, Fernando ; Scott, Janet ; Sharma, Ram. / Predicting ligand-free cell attachment on next generation cellulose-chitosan hydrogels. In: ACS OMEGA. 2018 ; Vol. 3, No. 1. pp. 937–945.
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