Predicting knot or catenane type of site-specific recombination products

Dorothy Buck; Erica Flapan

doi:10.1016/j.jmb.2007.10.016

Predicting knot or catenane type of site-specific recombination products

Dorothy Buck, Erica Flapan

Research output: Contribution to journal › Article › peer-review

28 Citations (SciVal)

Abstract

Site-specific recombination on supercoiled circular DNA yields a variety of knotted or catenated products. Here, we present a topological model of this process and characterize all possible products of the most common substrates: unknots, unlinks, and torus knots and catenanes. This model tightly prescribes the knot or catenane type of previously uncharacterized data. We also discuss how the model helps to distinguish products of distributive recombination and, in some cases, determine the order of processive recombination products.

Original language	English
Pages (from-to)	1186-99
Number of pages	14
Journal	Journal of Molecular Biology
Volume	374
Issue number	5
DOIs	https://doi.org/10.1016/j.jmb.2007.10.016
Publication status	Published - 14 Dec 2007

Keywords

DNA, Superhelical
Models, Molecular
Nucleic Acid Conformation
Recombination, Genetic
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

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10.1016/j.jmb.2007.10.016

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title = "Predicting knot or catenane type of site-specific recombination products",

abstract = "Site-specific recombination on supercoiled circular DNA yields a variety of knotted or catenated products. Here, we present a topological model of this process and characterize all possible products of the most common substrates: unknots, unlinks, and torus knots and catenanes. This model tightly prescribes the knot or catenane type of previously uncharacterized data. We also discuss how the model helps to distinguish products of distributive recombination and, in some cases, determine the order of processive recombination products.",

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author = "Dorothy Buck and Erica Flapan",

year = "2007",

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doi = "10.1016/j.jmb.2007.10.016",

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AU - Buck, Dorothy

AU - Flapan, Erica

PY - 2007/12/14

Y1 - 2007/12/14

N2 - Site-specific recombination on supercoiled circular DNA yields a variety of knotted or catenated products. Here, we present a topological model of this process and characterize all possible products of the most common substrates: unknots, unlinks, and torus knots and catenanes. This model tightly prescribes the knot or catenane type of previously uncharacterized data. We also discuss how the model helps to distinguish products of distributive recombination and, in some cases, determine the order of processive recombination products.

AB - Site-specific recombination on supercoiled circular DNA yields a variety of knotted or catenated products. Here, we present a topological model of this process and characterize all possible products of the most common substrates: unknots, unlinks, and torus knots and catenanes. This model tightly prescribes the knot or catenane type of previously uncharacterized data. We also discuss how the model helps to distinguish products of distributive recombination and, in some cases, determine the order of processive recombination products.

KW - DNA, Superhelical

KW - Models, Molecular

KW - Nucleic Acid Conformation

KW - Recombination, Genetic

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, Non-P.H.S.

U2 - 10.1016/j.jmb.2007.10.016

DO - 10.1016/j.jmb.2007.10.016

M3 - Article

C2 - 17996894

SN - 0022-2836

VL - 374

SP - 1186

EP - 1199

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 5

ER -

Predicting knot or catenane type of site-specific recombination products

Abstract

Keywords

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