Precision navigation of hepatic ischemia reperfusion injury guided by lysosomal viscosity-activatable NIR-II fluorescence

Jihong Liu; Wen Zhang; Chunmiao Zhou; Mengmei Li; Xin Wang; Wei Zhang; Zhenzhen Liu; Frank Wu; Tony James; Ping  Li; Bo Tang

doi:10.1021/jacs.2c03832

Precision navigation of hepatic ischemia reperfusion injury guided by lysosomal viscosity-activatable NIR-II fluorescence

Jihong Liu, Wen Zhang, Chunmiao Zhou, Mengmei Li, Xin Wang, Wei Zhang, Zhenzhen Liu, Frank Wu, Tony James, Ping Li, Bo Tang

Research output: Contribution to journal › Article › peer-review

85 Citations (SciVal)

Abstract

Hepatic ischemia-reperfusion injury (HIRI) is responsible for postoperative liver dysfunction and liver failure. Precise and rapid navigation of HIRI lesions is critical for early warning and timely development of pretreatment plans. Available methods for assaying liver injury fail to provide the exact location of lesions in real time intraoperatively. HIRI is intimately associated with oxidative stress which impairs lysosomal degradative function, leading to significant changes in lysosomal viscosity. Therefore, lysosomal viscosity is a potential biomarker for the precise targeting of HIRI. Hence, we developed a viscosity-activatable second near-infrared window fluorescent probe (NP-V) for the detection of lysosomal viscosity in hepatocytes and mice during HIRI. A reactive oxygen species-malondialdehyde-cathepsin B signaling pathway during HIRI was established. We further conducted high signal-to-background ratio NIR-II fluorescence imaging of HIRI mice. The contour and boundary of liver lesions were delineated, and as such the precise intraoperative resection of the lesion area was implemented. This research demonstrates the potential of NP-V as a dual-functional probe for the elucidation of HIRI pathogenesis and the direct navigation of HIRI lesions in clinical applications.

Original language	English
Pages (from-to)	13586-13599
Number of pages	14
Journal	Journal of the American Chemical Society
Volume	144
Issue number	30
Early online date	6 Jul 2022
DOIs	https://doi.org/10.1021/jacs.2c03832
Publication status	Published - 3 Aug 2022

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (22134004, 21927811, 22074083, and 22077075), the Key Research and Development Program of Shandong Province (2018YFJH0502), the National Science Foundation of Shandong Province of China (ZR2020ZD17), and Local Science and Technology Development Fund Guided by the Central Government (YDZX20193700002026, YDZX20203700002568). T.D.J. wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). L.W. wishes to thank the China Scholarship Council and the University of Bath for supporting his Ph.D. in the U.K.

Publisher Copyright:
© 2022 The Authors. Published by American Chemical Society.

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/jacs.2c03832Licence: CC BY

Cite this

@article{42f4a19c1d43439e85f54b78e123750b,

title = "Precision navigation of hepatic ischemia reperfusion injury guided by lysosomal viscosity-activatable NIR-II fluorescence",

abstract = "Hepatic ischemia-reperfusion injury (HIRI) is responsible for postoperative liver dysfunction and liver failure. Precise and rapid navigation of HIRI lesions is critical for early warning and timely development of pretreatment plans. Available methods for assaying liver injury fail to provide the exact location of lesions in real time intraoperatively. HIRI is intimately associated with oxidative stress which impairs lysosomal degradative function, leading to significant changes in lysosomal viscosity. Therefore, lysosomal viscosity is a potential biomarker for the precise targeting of HIRI. Hence, we developed a viscosity-activatable second near-infrared window fluorescent probe (NP-V) for the detection of lysosomal viscosity in hepatocytes and mice during HIRI. A reactive oxygen species-malondialdehyde-cathepsin B signaling pathway during HIRI was established. We further conducted high signal-to-background ratio NIR-II fluorescence imaging of HIRI mice. The contour and boundary of liver lesions were delineated, and as such the precise intraoperative resection of the lesion area was implemented. This research demonstrates the potential of NP-V as a dual-functional probe for the elucidation of HIRI pathogenesis and the direct navigation of HIRI lesions in clinical applications.",

author = "Jihong Liu and Wen Zhang and Chunmiao Zhou and Mengmei Li and Xin Wang and Wei Zhang and Zhenzhen Liu and Frank Wu and Tony James and Ping Li and Bo Tang",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (22134004, 21927811, 22074083, and 22077075), the Key Research and Development Program of Shandong Province (2018YFJH0502), the National Science Foundation of Shandong Province of China (ZR2020ZD17), and Local Science and Technology Development Fund Guided by the Central Government (YDZX20193700002026, YDZX20203700002568). T.D.J. wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). L.W. wishes to thank the China Scholarship Council and the University of Bath for supporting his Ph.D. in the U.K. Publisher Copyright: {\textcopyright} 2022 The Authors. Published by American Chemical Society.",

year = "2022",

month = aug,

day = "3",

doi = "10.1021/jacs.2c03832",

language = "English",

volume = "144",

pages = "13586--13599",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "30",

}

TY - JOUR

T1 - Precision navigation of hepatic ischemia reperfusion injury guided by lysosomal viscosity-activatable NIR-II fluorescence

AU - Liu, Jihong

AU - Zhang, Wen

AU - Zhou, Chunmiao

AU - Li, Mengmei

AU - Wang, Xin

AU - Zhang, Wei

AU - Liu, Zhenzhen

AU - Wu, Frank

AU - James, Tony

AU - Li, Ping

AU - Tang, Bo

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (22134004, 21927811, 22074083, and 22077075), the Key Research and Development Program of Shandong Province (2018YFJH0502), the National Science Foundation of Shandong Province of China (ZR2020ZD17), and Local Science and Technology Development Fund Guided by the Central Government (YDZX20193700002026, YDZX20203700002568). T.D.J. wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). L.W. wishes to thank the China Scholarship Council and the University of Bath for supporting his Ph.D. in the U.K. Publisher Copyright: © 2022 The Authors. Published by American Chemical Society.

PY - 2022/8/3

Y1 - 2022/8/3

N2 - Hepatic ischemia-reperfusion injury (HIRI) is responsible for postoperative liver dysfunction and liver failure. Precise and rapid navigation of HIRI lesions is critical for early warning and timely development of pretreatment plans. Available methods for assaying liver injury fail to provide the exact location of lesions in real time intraoperatively. HIRI is intimately associated with oxidative stress which impairs lysosomal degradative function, leading to significant changes in lysosomal viscosity. Therefore, lysosomal viscosity is a potential biomarker for the precise targeting of HIRI. Hence, we developed a viscosity-activatable second near-infrared window fluorescent probe (NP-V) for the detection of lysosomal viscosity in hepatocytes and mice during HIRI. A reactive oxygen species-malondialdehyde-cathepsin B signaling pathway during HIRI was established. We further conducted high signal-to-background ratio NIR-II fluorescence imaging of HIRI mice. The contour and boundary of liver lesions were delineated, and as such the precise intraoperative resection of the lesion area was implemented. This research demonstrates the potential of NP-V as a dual-functional probe for the elucidation of HIRI pathogenesis and the direct navigation of HIRI lesions in clinical applications.

AB - Hepatic ischemia-reperfusion injury (HIRI) is responsible for postoperative liver dysfunction and liver failure. Precise and rapid navigation of HIRI lesions is critical for early warning and timely development of pretreatment plans. Available methods for assaying liver injury fail to provide the exact location of lesions in real time intraoperatively. HIRI is intimately associated with oxidative stress which impairs lysosomal degradative function, leading to significant changes in lysosomal viscosity. Therefore, lysosomal viscosity is a potential biomarker for the precise targeting of HIRI. Hence, we developed a viscosity-activatable second near-infrared window fluorescent probe (NP-V) for the detection of lysosomal viscosity in hepatocytes and mice during HIRI. A reactive oxygen species-malondialdehyde-cathepsin B signaling pathway during HIRI was established. We further conducted high signal-to-background ratio NIR-II fluorescence imaging of HIRI mice. The contour and boundary of liver lesions were delineated, and as such the precise intraoperative resection of the lesion area was implemented. This research demonstrates the potential of NP-V as a dual-functional probe for the elucidation of HIRI pathogenesis and the direct navigation of HIRI lesions in clinical applications.

UR - http://www.scopus.com/inward/record.url?scp=85135596749&partnerID=8YFLogxK

U2 - 10.1021/jacs.2c03832

DO - 10.1021/jacs.2c03832

M3 - Article

SN - 0002-7863

VL - 144

SP - 13586

EP - 13599

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 30

ER -

Precision navigation of hepatic ischemia reperfusion injury guided by lysosomal viscosity-activatable NIR-II fluorescence

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this