We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60 ng/ml with<7% precision in 13 min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08 ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22 min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology.
- miniaturised ELISA
- smartphone readout
- point of care diagnostics
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- Department of Chemical Engineering - Reader
- Centre for Biosensors, Bioelectronics and Biodevices (C3Bio)
- EPSRC Centre for Doctoral Training in Statistical Applied Mathematics (SAMBa)
- Water Innovation and Research Centre (WIRC)
- Centre for Sustainable and Circular Technologies (CSCT)
- Reaction and Catalysis Engineering research unit (RaCE)
- Centre for Integrated Bioprocessing Research (CIBR)
Person: Research & Teaching