Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device

Ana I. Barbosa, Poonam Gehlot, Kalpita Sidapra, Alexander D. Edwards, Nuno M. Reis

Research output: Contribution to journalArticle

194 Citations (SciVal)


We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60 ng/ml with<7% precision in 13 min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08 ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22 min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology.
Original languageEnglish
Pages (from-to)5-14
Number of pages10
JournalBiosensors and Bioelectronics
Early online date5 Mar 2015
Publication statusPublished - 15 Aug 2015


  • microfluidic
  • miniaturised ELISA
  • smartphone readout
  • fluorescence
  • Colorimetric
  • point of care diagnostics


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