Polymer Indicator Displacement Assay: Electrochemical Glucose Monitoring Based on Boronic Acid Receptors and Graphene Foam Competitively Binding with Poly-Nordihydroguaiaretic Acid

Simon Wikeley, Jakub Przybylowski, Pablo Lozano-Sanchez , Marco Caffio , Tony James, Steven Bull, Philip Fletcher, Frank Marken

Research output: Contribution to journalArticlepeer-review

14 Downloads (Pure)

Abstract

The concept of a reversible polymer displacement sensor mechanism for electrochemical glucose monitoring is demonstrated. A pyrene-derivatised boronic acid chemo-receptor for glucose is adsorbed onto a graphene foam electrode. Spontaneous oxidative polymerisation of nordihydroguaiaretic acid (NHG) onto the graphene foam electrode leads to a redox active film (poly-NHG) covalently attached to the boronic acid receptors. Oxidation of poly-NHG frees the boronic acid receptors to interact with glucose from the solution phase, which is detected due to competitive binding when reduced poly-NHG re-binds to the boronic acid functional groups. The sensor shows the anticipated boronic acid selectivity of fructose > glucose. The ratio of charges under the voltammetric peaks for poly-NHG unbound and bound is employed for glucose sensing with an approximately linear analytical range from 1 to 50 mM glucose in aqueous pH 7 buffer. The new methodology is shown to give apparent saccharide – boronic acid binding constants and to work in human serum. Therefore, in the future it could be developed further for glucose monitoring.
Original languageEnglish
Pages (from-to)661-670
Number of pages10
JournalThe Analyst
Volume147
Issue number4
Early online date19 Jan 2022
DOIs
Publication statusPublished - 21 Feb 2022

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Fingerprint

Dive into the research topics of 'Polymer Indicator Displacement Assay: Electrochemical Glucose Monitoring Based on Boronic Acid Receptors and Graphene Foam Competitively Binding with Poly-Nordihydroguaiaretic Acid'. Together they form a unique fingerprint.

Cite this