Platelet lysate gel and endothelial progenitors stimulate microvascular network formation in vitro: tissue engineering implications

Tiago Fortunato, Cristina Beltrami, Costanza Emanueli, Paul De Bank, Giordano Pula

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Abstract

Revascularisation is a key step for tissue regeneration and complete organ engineering. We describe the generation of human platelet lysate gel (hPLG), an extracellular matrix preparation from human platelets able to support the proliferation of endothelial colony forming cells (ECFCs) in 2D cultures and the formation of a complete microvascular network in vitro in 3D cultures. Existing extracellular matrix preparations require addition of high concentrations of recombinant growth factors and allow only limited formation of capillary-like structures. Additional advantages of our approach over existing extracellular matrices are the absence of any animal product in the composition hPLG and the possibility of obtaining hPLG from patients to generate homologous scaffolds for re-implantation. This discovery has the potential to accelerate the development of regenerative medicine applications based on implantation of microvascular networks expanded ex vivo or the generation of fully vascularised organs.
Original languageEnglish
Article number25326
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 4 May 2016

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Tissue Engineering
Microvessels
Blood Platelets
Gels
Extracellular Matrix
Regenerative Medicine
Regeneration
Intercellular Signaling Peptides and Proteins
In Vitro Techniques

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Platelet lysate gel and endothelial progenitors stimulate microvascular network formation in vitro: tissue engineering implications. / Fortunato, Tiago; Beltrami, Cristina; Emanueli, Costanza; De Bank, Paul; Pula, Giordano.

In: Scientific Reports, Vol. 6, 25326, 04.05.2016.

Research output: Contribution to journalArticle

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