A method based on plasmon resonance Rayleigh scattering (PRRS) spectroscopy and dark-field microscopy (DFM) was established for the real-time monitoring of a click reaction at the single-nanoparticle level. Click reactions on the surface of single gold nanoparticles (GNPs) result in interparticle coupling, which leads to a red-shift of the λ (Δλ =43nm) in the PRRS spectra and a color change of the single gold nanoparticles in DFM (from green to orange).
Shi, L., Jing, C., Ma, W., Li, D-W., Halls, J. E., Marken, F., & Long, Y-T. (2013). Plasmon resonance scattering spectroscopy at the single-nanoparticle level: Real-time monitoring of a click reaction. Angewandte Chemie-International Edition, 52(23), 6011-6014. https://doi.org/10.1002/anie.201301930