Plasmid profile analysis of Portuguese Borrelia lusitaniae strains

L Vitorino; Gabriele Margos; L Ze-Ze; Klaus Kurtenbach; M Collares-Pereira

doi:10.1016/j.ttbdis.2010.07.001

Plasmid profile analysis of Portuguese Borrelia lusitaniae strains

L Vitorino, Gabriele Margos, L Ze-Ze, Klaus Kurtenbach, M Collares-Pereira

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

Plasmid profiles of 2 Portuguese Borrelia lusitaniae strains, one isolated from a human patient and the other one from an Ixodes ricinus tick, were obtained by pulsed-field gel electrophoresis to evaluate the plasmid diversity in each strain. Overall, a maximum of 6 plasmids were detected that ranged from 19 kb to 76 kb, revealing completely different plasmid profiles from those previously described for other genospecies of B. burgdorferi sensu lato, the causative agents of Lyme borreliosis. The plasmid location of the ospA gene was investigated by hybridization, allowing its allocation to the plasmid of 70 kb instead of the 54 kb linear plasmid described for B. burgdorferi sensu stricto strains.

Original language	English
Pages (from-to)	125-128
Number of pages	4
Journal	Ticks and Tick-Borne Diseases
Volume	1
Issue number	3
DOIs	https://doi.org/10.1016/j.ttbdis.2010.07.001
Publication status	Published - Sept 2010

Keywords

Lyme borreliosis
ospA
genome organization
Borrelia lusitaniae
ixodes ricinus

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10.1016/j.ttbdis.2010.07.001

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title = "Plasmid profile analysis of Portuguese Borrelia lusitaniae strains",

abstract = "Plasmid profiles of 2 Portuguese Borrelia lusitaniae strains, one isolated from a human patient and the other one from an Ixodes ricinus tick, were obtained by pulsed-field gel electrophoresis to evaluate the plasmid diversity in each strain. Overall, a maximum of 6 plasmids were detected that ranged from 19 kb to 76 kb, revealing completely different plasmid profiles from those previously described for other genospecies of B. burgdorferi sensu lato, the causative agents of Lyme borreliosis. The plasmid location of the ospA gene was investigated by hybridization, allowing its allocation to the plasmid of 70 kb instead of the 54 kb linear plasmid described for B. burgdorferi sensu stricto strains.",

keywords = "Lyme borreliosis, ospA, genome organization, Borrelia lusitaniae, ixodes ricinus",

author = "L Vitorino and Gabriele Margos and L Ze-Ze and Klaus Kurtenbach and M Collares-Pereira",

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doi = "10.1016/j.ttbdis.2010.07.001",

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T1 - Plasmid profile analysis of Portuguese Borrelia lusitaniae strains

AU - Vitorino, L

AU - Margos, Gabriele

AU - Ze-Ze, L

AU - Kurtenbach, Klaus

AU - Collares-Pereira, M

PY - 2010/9

Y1 - 2010/9

N2 - Plasmid profiles of 2 Portuguese Borrelia lusitaniae strains, one isolated from a human patient and the other one from an Ixodes ricinus tick, were obtained by pulsed-field gel electrophoresis to evaluate the plasmid diversity in each strain. Overall, a maximum of 6 plasmids were detected that ranged from 19 kb to 76 kb, revealing completely different plasmid profiles from those previously described for other genospecies of B. burgdorferi sensu lato, the causative agents of Lyme borreliosis. The plasmid location of the ospA gene was investigated by hybridization, allowing its allocation to the plasmid of 70 kb instead of the 54 kb linear plasmid described for B. burgdorferi sensu stricto strains.

AB - Plasmid profiles of 2 Portuguese Borrelia lusitaniae strains, one isolated from a human patient and the other one from an Ixodes ricinus tick, were obtained by pulsed-field gel electrophoresis to evaluate the plasmid diversity in each strain. Overall, a maximum of 6 plasmids were detected that ranged from 19 kb to 76 kb, revealing completely different plasmid profiles from those previously described for other genospecies of B. burgdorferi sensu lato, the causative agents of Lyme borreliosis. The plasmid location of the ospA gene was investigated by hybridization, allowing its allocation to the plasmid of 70 kb instead of the 54 kb linear plasmid described for B. burgdorferi sensu stricto strains.

KW - Lyme borreliosis

KW - ospA

KW - genome organization

KW - Borrelia lusitaniae

KW - ixodes ricinus

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UR - http://dx.doi.org/10.1016/j.ttbdis.2010.07.001

U2 - 10.1016/j.ttbdis.2010.07.001

DO - 10.1016/j.ttbdis.2010.07.001

M3 - Article

SN - 1877-959X

VL - 1

SP - 125

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JO - Ticks and Tick-Borne Diseases

JF - Ticks and Tick-Borne Diseases

IS - 3

ER -

Plasmid profile analysis of Portuguese Borrelia lusitaniae strains

Abstract

Keywords

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