Plasmid profile analysis of Portuguese Borrelia lusitaniae strains

L Vitorino, Gabriele Margos, L Ze-Ze, Klaus Kurtenbach, M Collares-Pereira

Research output: Contribution to journalArticle

Abstract

Plasmid profiles of 2 Portuguese Borrelia lusitaniae strains, one isolated from a human patient and the other one from an Ixodes ricinus tick, were obtained by pulsed-field gel electrophoresis to evaluate the plasmid diversity in each strain. Overall, a maximum of 6 plasmids were detected that ranged from 19 kb to 76 kb, revealing completely different plasmid profiles from those previously described for other genospecies of B. burgdorferi sensu lato, the causative agents of Lyme borreliosis. The plasmid location of the ospA gene was investigated by hybridization, allowing its allocation to the plasmid of 70 kb instead of the 54 kb linear plasmid described for B. burgdorferi sensu stricto strains.
Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalTicks and Tick-Borne Diseases
Volume1
Issue number3
DOIs
Publication statusPublished - Sep 2010

Keywords

  • Lyme borreliosis
  • ospA
  • genome organization
  • Borrelia lusitaniae
  • ixodes ricinus

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    Vitorino, L., Margos, G., Ze-Ze, L., Kurtenbach, K., & Collares-Pereira, M. (2010). Plasmid profile analysis of Portuguese Borrelia lusitaniae strains. Ticks and Tick-Borne Diseases, 1(3), 125-128. https://doi.org/10.1016/j.ttbdis.2010.07.001