Plasma membrane expression of GnRH receptors: regulation by antagonists in breast, prostate, and gonadotrope cell lines.

Ann R Finch; Kathleen R Sedgley; Christopher J Caunt; Craig A McArdle

doi:10.1677/JOE-07-0471

Plasma membrane expression of GnRH receptors: regulation by antagonists in breast, prostate, and gonadotrope cell lines.

Ann R Finch
, Kathleen R Sedgley
, Christopher J Caunt
, Craig A McArdle

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

45 Citations (SciVal)

Abstract

In heterologous expression systems, human GnRH receptors (hGnRHRs) are poorly expressed at the cell surface and this may reflect inefficient exit from the endoplasmic reticulum. Here, we have defined the proportion of GnRHRs at the cell surface using a novel assay based on adenoviral transduction with epitope-tagged GnRHRs followed by staining and semi-automated imaging. We find that in MCF7 (breast cancer) cells, the proportional cell surface expression (PCSE) of hGnRHRs is remarkably low (

Original language	English
Pages (from-to)	353-367
Number of pages	15
Journal	The Journal of Endocrinology
Volume	196
Issue number	2
DOIs	https://doi.org/10.1677/JOE-07-0471
Publication status	Published - 2008

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10.1677/JOE-07-0471

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abstract = "In heterologous expression systems, human GnRH receptors (hGnRHRs) are poorly expressed at the cell surface and this may reflect inefficient exit from the endoplasmic reticulum. Here, we have defined the proportion of GnRHRs at the cell surface using a novel assay based on adenoviral transduction with epitope-tagged GnRHRs followed by staining and semi-automated imaging. We find that in MCF7 (breast cancer) cells, the proportional cell surface expression (PCSE) of hGnRHRs is remarkably low (",

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AU - Finch, Ann R

AU - Sedgley, Kathleen R

AU - Caunt, Christopher J

AU - McArdle, Craig A

PY - 2008

Y1 - 2008

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AB - In heterologous expression systems, human GnRH receptors (hGnRHRs) are poorly expressed at the cell surface and this may reflect inefficient exit from the endoplasmic reticulum. Here, we have defined the proportion of GnRHRs at the cell surface using a novel assay based on adenoviral transduction with epitope-tagged GnRHRs followed by staining and semi-automated imaging. We find that in MCF7 (breast cancer) cells, the proportional cell surface expression (PCSE) of hGnRHRs is remarkably low (

UR - https://www.scopus.com/pages/publications/39749202910

UR - http://dx.doi.org/10.1677/JOE-07-0471

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DO - 10.1677/JOE-07-0471

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JF - The Journal of Endocrinology

IS - 2

ER -

Plasma membrane expression of GnRH receptors: regulation by antagonists in breast, prostate, and gonadotrope cell lines.

Abstract

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