Plasma membrane expression of GnRH receptors: regulation by antagonists in breast, prostate, and gonadotrope cell lines.

Ann R Finch, Kathleen R Sedgley, Christopher J Caunt, Craig A McArdle

Research output: Contribution to journalArticle

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Abstract

In heterologous expression systems, human GnRH receptors (hGnRHRs) are poorly expressed at the cell surface and this may reflect inefficient exit from the endoplasmic reticulum. Here, we have defined the proportion of GnRHRs at the cell surface using a novel assay based on adenoviral transduction with epitope-tagged GnRHRs followed by staining and semi-automated imaging. We find that in MCF7 (breast cancer) cells, the proportional cell surface expression (PCSE) of hGnRHRs is remarkably low (
LanguageEnglish
Pages353-367
Number of pages15
JournalThe Journal of Endocrinology
Volume196
Issue number2
DOIs
StatusPublished - 2008

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LHRH Receptors
Prostate
Breast
Cell Membrane
Cell Line
Endoplasmic Reticulum
Epitopes
Staining and Labeling
Breast Neoplasms

Cite this

Plasma membrane expression of GnRH receptors: regulation by antagonists in breast, prostate, and gonadotrope cell lines. / Finch, Ann R; Sedgley, Kathleen R; Caunt, Christopher J; McArdle, Craig A.

In: The Journal of Endocrinology, Vol. 196, No. 2, 2008, p. 353-367.

Research output: Contribution to journalArticle

Finch, Ann R ; Sedgley, Kathleen R ; Caunt, Christopher J ; McArdle, Craig A. / Plasma membrane expression of GnRH receptors: regulation by antagonists in breast, prostate, and gonadotrope cell lines.In: The Journal of Endocrinology. 2008 ; Vol. 196, No. 2. pp. 353-367
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