Plasma glucagon-like peptide-1 responses to ingestion of protein with increasing doses of milk minerals rich in calcium: Protein, calcium and GLP-1 release

Jon Watkins, Harry A. Smith, Lisa Hoj Brunsgaard, Ulla Ramer Mikkelsen, Francoise Koumanov, James A. Betts, Javier T. Gonzalez

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Abstract

A high dose of whey protein hydrolysate fed with milk minerals rich in calcium (Capolac®) results in enhanced glucagon-like peptide-1 (GLP-1) concentrations in lean individuals; however, the effect of different calcium doses ingested alongside protein is unknown. The present study assessed the dose response of calcium fed alongside 25 g whey protein hydrolysate on GLP-1 concentrations in individuals with overweight/obesity. Eighteen adults (mean ± sd: 8M/10F, 34 ± 18 years, 28·2 ± 2·9 kgm-2) completed four trials in a randomised, double-blind, crossover design. Participants consumed test solutions consisting of 25 g whey protein hydrolysate (CON), supplemented with 3179 mg (LOW), 6363 mg (MED) or 9547 mg (HIGH) Capolac® on different occasions, separated by at least 48 h. The calcium content of test solutions equated to 65, 892, 1719 and 2547 mg, respectively. Arterialised-venous blood was sampled over 180 min to determine plasma concentrations of GLP-1TOTAL, GLP-17-36amide, insulin, glucose, NEFA, and serum concentrations of calcium and albumin. Ad libitum energy intake was measured at 180 min. Time-averaged incremental AUC (iAUC) for GLP-1TOTAL (pmol·l-1·min-1) did not differ between CON (23 ± 4), LOW (25 ± 6), MED (24 ± 5) and HIGH (24 ± 6). Energy intake (kcal) did not differ between CON (940 ± 387), LOW (884 ± 345), MED (920 ± 334) and HIGH (973 ± 390). Co-ingestion of whey protein hydrolysate with Capolac® does not potentiate GLP-1 release in comparison with whey protein hydrolysate alone. The study was registered at clinical trials (NCT03819972).

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalBritish Journal of Nutrition
Volume128
Issue number1
Early online date9 Aug 2021
DOIs
Publication statusPublished - 14 Jul 2022

Keywords

  • Appetite
  • Calcium
  • Energy intake
  • GLP-1
  • Protein

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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