Plasma glucagon-like peptide-1 responses to ingestion of protein with increasing doses of milk minerals rich in calcium: Protein, calcium and GLP-1 release

Jon Watkins; Harry A. Smith; Lisa Hoj Brunsgaard; Ulla Ramer Mikkelsen; Francoise Koumanov; James A. Betts; Javier T. Gonzalez

doi:10.1017/S000711452100297X

Plasma glucagon-like peptide-1 responses to ingestion of protein with increasing doses of milk minerals rich in calcium: Protein, calcium and GLP-1 release

Jon Watkins, Harry A. Smith, Lisa Hoj Brunsgaard, Ulla Ramer Mikkelsen, Francoise Koumanov, James A. Betts, Javier T. Gonzalez

ARLA Foods Ingredients

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Abstract

A high dose of whey protein hydrolysate fed with milk minerals rich in calcium (Capolac®) results in enhanced glucagon-like peptide-1 (GLP-1) concentrations in lean individuals; however, the effect of different calcium doses ingested alongside protein is unknown. The present study assessed the dose response of calcium fed alongside 25 g whey protein hydrolysate on GLP-1 concentrations in individuals with overweight/obesity. Eighteen adults (mean ± sd: 8M/10F, 34 ± 18 years, 28·2 ± 2·9 kgm-2) completed four trials in a randomised, double-blind, crossover design. Participants consumed test solutions consisting of 25 g whey protein hydrolysate (CON), supplemented with 3179 mg (LOW), 6363 mg (MED) or 9547 mg (HIGH) Capolac® on different occasions, separated by at least 48 h. The calcium content of test solutions equated to 65, 892, 1719 and 2547 mg, respectively. Arterialised-venous blood was sampled over 180 min to determine plasma concentrations of GLP-1TOTAL, GLP-17-36amide, insulin, glucose, NEFA, and serum concentrations of calcium and albumin. Ad libitum energy intake was measured at 180 min. Time-averaged incremental AUC (iAUC) for GLP-1TOTAL (pmol·l-1·min-1) did not differ between CON (23 ± 4), LOW (25 ± 6), MED (24 ± 5) and HIGH (24 ± 6). Energy intake (kcal) did not differ between CON (940 ± 387), LOW (884 ± 345), MED (920 ± 334) and HIGH (973 ± 390). Co-ingestion of whey protein hydrolysate with Capolac® does not potentiate GLP-1 release in comparison with whey protein hydrolysate alone. The study was registered at clinical trials (NCT03819972).

Original language	English
Pages (from-to)	55-63
Number of pages	9
Journal	British Journal of Nutrition
Volume	128
Issue number	1
Early online date	9 Aug 2021
DOIs	https://doi.org/10.1017/S000711452100297X
Publication status	Published - 14 Jul 2022

Bibliographical note

Funding Information:
J. T. G. is a named investigator on research grants funded by BBSRC, MRC, British Heart Foundation, Lucozade Ribena Suntory, Arla Foods Ingredients, Kenniscentrum Suiker and Voeding and has received paid consultancy for PepsiCo, Lucozade Ribena Suntory and SVGC. J. A. B. is a named investigator on research grants funded by BBSRC, MRC, British Heart Foundation, Rare Disease Foundation, EU Hydration Institute, GlaxoSmithKline, Nestlé, Lucozade Ribena Suntory, ARLA Foods and Kennis Centrum Suiker; has completed paid consultancy for PepsiCo, Kellogg’s and SVGC; receives an annual stipend as Editor-in Chief of International Journal of Sport Nutrition & Exercise Metabolism; and receives an annual honorarium as a member of the academic advisory board for the International Olympic Committee Diploma in Sports Nutrition.

Funding Information:
The authors thank the volunteers for their time and effort in participating in this study. This work was funded by Arla Foods Ingredients and the University of Bath.

Publisher Copyright:
© 2022 Cambridge University Press. All rights reserved.

Keywords

Appetite
Calcium
Energy intake
GLP-1
Protein

ASJC Scopus subject areas

Medicine (miscellaneous)
Nutrition and Dietetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1017/S000711452100297X

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Cite this

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title = "Plasma glucagon-like peptide-1 responses to ingestion of protein with increasing doses of milk minerals rich in calcium: Protein, calcium and GLP-1 release",

abstract = "A high dose of whey protein hydrolysate fed with milk minerals rich in calcium (Capolac{\textregistered}) results in enhanced glucagon-like peptide-1 (GLP-1) concentrations in lean individuals; however, the effect of different calcium doses ingested alongside protein is unknown. The present study assessed the dose response of calcium fed alongside 25 g whey protein hydrolysate on GLP-1 concentrations in individuals with overweight/obesity. Eighteen adults (mean ± sd: 8M/10F, 34 ± 18 years, 28·2 ± 2·9 kgm-2) completed four trials in a randomised, double-blind, crossover design. Participants consumed test solutions consisting of 25 g whey protein hydrolysate (CON), supplemented with 3179 mg (LOW), 6363 mg (MED) or 9547 mg (HIGH) Capolac{\textregistered} on different occasions, separated by at least 48 h. The calcium content of test solutions equated to 65, 892, 1719 and 2547 mg, respectively. Arterialised-venous blood was sampled over 180 min to determine plasma concentrations of GLP-1TOTAL, GLP-17-36amide, insulin, glucose, NEFA, and serum concentrations of calcium and albumin. Ad libitum energy intake was measured at 180 min. Time-averaged incremental AUC (iAUC) for GLP-1TOTAL (pmol·l-1·min-1) did not differ between CON (23 ± 4), LOW (25 ± 6), MED (24 ± 5) and HIGH (24 ± 6). Energy intake (kcal) did not differ between CON (940 ± 387), LOW (884 ± 345), MED (920 ± 334) and HIGH (973 ± 390). Co-ingestion of whey protein hydrolysate with Capolac{\textregistered} does not potentiate GLP-1 release in comparison with whey protein hydrolysate alone. The study was registered at clinical trials (NCT03819972).",

keywords = "Appetite, Calcium, Energy intake, GLP-1, Protein",

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note = "Funding Information: J. T. G. is a named investigator on research grants funded by BBSRC, MRC, British Heart Foundation, Lucozade Ribena Suntory, Arla Foods Ingredients, Kenniscentrum Suiker and Voeding and has received paid consultancy for PepsiCo, Lucozade Ribena Suntory and SVGC. J. A. B. is a named investigator on research grants funded by BBSRC, MRC, British Heart Foundation, Rare Disease Foundation, EU Hydration Institute, GlaxoSmithKline, Nestl{\'e}, Lucozade Ribena Suntory, ARLA Foods and Kennis Centrum Suiker; has completed paid consultancy for PepsiCo, Kellogg{\textquoteright}s and SVGC; receives an annual stipend as Editor-in Chief of International Journal of Sport Nutrition & Exercise Metabolism; and receives an annual honorarium as a member of the academic advisory board for the International Olympic Committee Diploma in Sports Nutrition. Funding Information: The authors thank the volunteers for their time and effort in participating in this study. This work was funded by Arla Foods Ingredients and the University of Bath. Publisher Copyright: {\textcopyright} 2022 Cambridge University Press. All rights reserved.",

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Plasma glucagon-like peptide-1 responses to ingestion of protein with increasing doses of milk minerals rich in calcium: Protein, calcium and GLP-1 release

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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Role of Rab3 in Peripheral Tissue Insulin Resistance

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Plasma glucagon-like peptide-1 responses to ingestion of protein with increasing doses of milk minerals rich in calcium: Protein, calcium and GLP-1 release

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

Role of Rab3 in Peripheral Tissue Insulin Resistance

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RX4041 RXDaytona+ Analyser

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