Tissue engineering (TE) aims to repair or replace defective tissues with the help of therapeutic cells and bioactive molecules, including the use of biomaterials as scaffolds for growth. Scaffold materials can be synthetic or natural and there has been significant recent interest in the use of decellularized tissues.

The objective of this research is to develop plant-based scaffolds for bone TE which are biocompatible, stable and can be modified to promote cell attachment and reproduction. Grass is our first candidate due to its renewable and scalable properties, and its mechanical strength makes it suitable for bone TE.

Grass blades were pre-treated with ethanol and PBS before decellularization. Decellularized grass (DCG) was surface modified with polymeric biomaterials. Scanning electron microscopy evaluated any morphological changes after surface modifications. Water contact angle and Fourier-transform infrared spectroscopy confirmed surface modifications. MG63 cells were quantified using fluorescence by nuclei counting on ImageJ.

Pre-treatment with absolute ethanol provided the highest level of decellularization and the morphological structure did not change after modification. FT-IR did not present obvious characterizations of modification materials due to the thinness of coating. Surface hydrophilicity was significantly increased after coating with poly(dopamine) (PDA), chitosan (CS) and PDA-CS. Fluorescent staining demonstrated promising adhesion of MG63 cells on DCG, which reached the same density as cells on tissue culture plastic. Data from proliferation experiments showed steady growth of MG63 cells on each sample except a slightly lower growth rate on PDA-CS and chitosan coated DCG.

This research indicates a good suitability for DCG as a biocompatible and renewable scaffold for MG63 cells and bone TE applications. In the future, surface functionalization of DCG with different biomolecules will be assessed and MG63 cell differentiation will be examined.
Original languageEnglish
Publication statusPublished - 15 Jun 2022
EventTissue and Cell Engineering Society Conference 2022 - University of Birmingham, Birmingham, UK United Kingdom
Duration: 13 Jun 202215 Jun 2022


ConferenceTissue and Cell Engineering Society Conference 2022
Abbreviated titleTCES 2022
Country/TerritoryUK United Kingdom


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