Pinkment: A synthetic platform for the development of fluorescent probes for diagnostic and theranostic applications

Maria Weber, Hai Hao Han, Bo Han Li, Maria L. Odyniec, Charlotte E.F. Jarman, Yi Zang, Steven D. Bull, Amanda B. Mackenzie, Adam C. Sedgwick, Jia Li, Xiao Peng He, Tony D. James

Research output: Contribution to journalArticle

Abstract

Reaction-based fluorescent-probes have proven successful for the visualisation of biological species in various cellular processes. Unfortunately, in order to tailor the design of a fluorescent probe to a specific application (i.e. organelle targeting, material and theranostic applications) often requires extensive synthetic efforts and the synthetic screening of a range of fluorophores to match the required synthetic needs. In this work, we have identified Pinkment-OH as a unique "plug-and-play"synthetic platform that can be used to develop a range of ONOO- responsive fluorescent probes for a variety of applications. These include theranostic-based applications and potential material-based/bioconjugation applications. The as prepared probes displayed an excellent sensitivity and selectivity for ONOO- over other ROS. In vitro studies using HeLa cells and RAW 264.7 macrophages demonstrated their ability to detect exogenously and endogenously produced ONOO-. Evaluation in an LPS-induced inflammation mouse model illustrated the ability to monitor ONOO- production in acute inflammation. Lastly, theranostic-based probes enabled the simultaneous evaluation of indomethacin-based therapeutic effects combined with the visualisation of an inflammation biomarker in RAW 264.7 cells. This journal is

Original languageEnglish
Pages (from-to)8567-8571
Number of pages5
JournalChemical Science
Volume11
Issue number32
Early online date6 Aug 2020
DOIs
Publication statusPublished - 21 Aug 2020

ASJC Scopus subject areas

  • Chemistry(all)

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