Pig cloning by microinjection of fetal fibroblast nuclei

A Onishi, M Iwamoto, T Akita, S Mikawa, K Takeda, T Awata, H Hanada, Anthony C F Perry

Research output: Contribution to journalArticle

674 Citations (Scopus)

Abstract

Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.
LanguageEnglish
Pages1188-1190
Number of pages3
JournalScience
Volume289
Issue number5482
StatusPublished - 2000

Fingerprint

fibroblasts
molecular cloning
swine
piglets
xenotransplantation
Sus scrofa
meat production
provenance
embryo (animal)
oocytes
microsatellite repeats
clones
color
DNA
cells

Cite this

Onishi, A., Iwamoto, M., Akita, T., Mikawa, S., Takeda, K., Awata, T., ... Perry, A. C. F. (2000). Pig cloning by microinjection of fetal fibroblast nuclei. Science, 289(5482), 1188-1190.

Pig cloning by microinjection of fetal fibroblast nuclei. / Onishi, A; Iwamoto, M; Akita, T; Mikawa, S; Takeda, K; Awata, T; Hanada, H; Perry, Anthony C F.

In: Science, Vol. 289, No. 5482, 2000, p. 1188-1190.

Research output: Contribution to journalArticle

Onishi, A, Iwamoto, M, Akita, T, Mikawa, S, Takeda, K, Awata, T, Hanada, H & Perry, ACF 2000, 'Pig cloning by microinjection of fetal fibroblast nuclei', Science, vol. 289, no. 5482, pp. 1188-1190.
Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T et al. Pig cloning by microinjection of fetal fibroblast nuclei. Science. 2000;289(5482):1188-1190.
Onishi, A ; Iwamoto, M ; Akita, T ; Mikawa, S ; Takeda, K ; Awata, T ; Hanada, H ; Perry, Anthony C F. / Pig cloning by microinjection of fetal fibroblast nuclei. In: Science. 2000 ; Vol. 289, No. 5482. pp. 1188-1190.
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