Physicochemical and in vitro biological evaluation of an injectable self-healing quaternized chitosan/oxidized pectin hydrogel for potential use as a wound dressing material

Mueanchan Chanmontri, Ahmed Swilem, Alexandra L. Mutch, Lisbeth Grøndahl, Orawan Suwantong

Research output: Contribution to journalArticlepeer-review

15 Citations (SciVal)

Abstract

Injectable self-healing hydrogels are attractive materials for use as wound dressings. To prepare such hydrogels, the current study used quaternized chitosan (QCS) to improve the solubility and antibacterial activity and oxidized pectin (OPEC) to introduce aldehyde groups for Schiff's base reaction with the amine groups from QCS. Self-healing hydrogels were made by co-injection of polymer solutions at specific polymer concentrations and reagent ratios that optimized both Schiff's base reactions and ionic interactions. The optimal hydrogel displayed self-healing 30 min after cutting and continuous self-healing during continuous step strain analysis, rapid gelation (< 1 min), a storage modulus of 394 Pa, and hardness of 700 mN, and compressibility of 162 mN s. The adhesiveness of this hydrogel (133 Pa) was within a suitable range for application as a wound dressing. The extraction media from the hydrogel displayed no cytotoxicity to NCTC clone 929 cells and higher cell migration than the control. While the extraction media from the hydrogel was found not to have antibacterial properties, QCS was verified as having MIC50 of 0.04 mg/mL against both E. coli and S. aureus. Therefore, this injectable self-healing QCS/OPEC hydrogel has the potential use as a biocompatible hydrogel material for wound management.

Original languageEnglish
Article number124984
JournalInternational Journal of Biological Macromolecules
Volume242
Early online date26 May 2023
DOIs
Publication statusPublished - 1 Jul 2023

Keywords

  • Injectable self-healing hydrogel
  • Oxidized pectin
  • Quaternized chitosan

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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