Phylogenetic analysis of transformable strains of thermophilic Bacillus species

David J. Studholme, Robin A. Jackson, David J. Leak

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Few strains of thermophilic Bacillus spp are readily transformable with plasmid DNA. Given the considerable phylogenetic and phenotypic diversity amongst thermophilic bacilli, we have examined whether transformability is a trait associated with a particular phylogenetic group, by sequencing the 16S ribosomal RNA genes from transformable strains NUB3621, K1041, and NRRL1174. Although all of these strains were described in the literature as B. stearothermophilus, only NRRL1174 is closely related to the type strain of this species. Based on its 16S rDNA sequence and physiological data K1041 appeared to belong to the species B. thermodenitrificans, while NUB3621 showed a slightly closer relationship to B. thermoglucosidasius than to B. stearothermophilus. Therefore we conclude that the trait of transformability, though possibly strain-specific, is not limited to a single species of thermophilic Bacillus. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalFEMS Microbiology Letters
Volume172
Issue number1
DOIs
Publication statusPublished - 1 Mar 1999

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Bacillus
16S Ribosomal RNA
Ribosomal DNA
rRNA Genes
Plasmids
DNA

Keywords

  • 16S rDNA
  • Bacillus stearothermophilus
  • Phylogeny
  • Thermophile

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Phylogenetic analysis of transformable strains of thermophilic Bacillus species. / Studholme, David J.; Jackson, Robin A.; Leak, David J.

In: FEMS Microbiology Letters, Vol. 172, No. 1, 01.03.1999, p. 85-90.

Research output: Contribution to journalArticle

Studholme, David J. ; Jackson, Robin A. ; Leak, David J. / Phylogenetic analysis of transformable strains of thermophilic Bacillus species. In: FEMS Microbiology Letters. 1999 ; Vol. 172, No. 1. pp. 85-90.
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