Phylogenetic analysis of transformable strains of thermophilic Bacillus species

David J. Studholme; Robin A. Jackson; David J. Leak

doi:10.1016/S0378-1097(99)00019-1

Phylogenetic analysis of transformable strains of thermophilic Bacillus species

David J. Studholme, Robin A. Jackson, David J. Leak

Imperial College London

Research output: Contribution to journal › Article › peer-review

33 Citations (SciVal)

Abstract

Few strains of thermophilic Bacillus spp are readily transformable with plasmid DNA. Given the considerable phylogenetic and phenotypic diversity amongst thermophilic bacilli, we have examined whether transformability is a trait associated with a particular phylogenetic group, by sequencing the 16S ribosomal RNA genes from transformable strains NUB3621, K1041, and NRRL1174. Although all of these strains were described in the literature as B. stearothermophilus, only NRRL1174 is closely related to the type strain of this species. Based on its 16S rDNA sequence and physiological data K1041 appeared to belong to the species B. thermodenitrificans, while NUB3621 showed a slightly closer relationship to B. thermoglucosidasius than to B. stearothermophilus. Therefore we conclude that the trait of transformability, though possibly strain-specific, is not limited to a single species of thermophilic Bacillus. Copyright (C) 1999 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	85-90
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	172
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(99)00019-1
Publication status	Published - 1 Mar 1999

Keywords

16S rDNA
Bacillus stearothermophilus
Phylogeny
Thermophile

ASJC Scopus subject areas

Genetics
Molecular Biology
Applied Microbiology and Biotechnology
Microbiology

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10.1016/S0378-1097(99)00019-1

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title = "Phylogenetic analysis of transformable strains of thermophilic Bacillus species",

abstract = "Few strains of thermophilic Bacillus spp are readily transformable with plasmid DNA. Given the considerable phylogenetic and phenotypic diversity amongst thermophilic bacilli, we have examined whether transformability is a trait associated with a particular phylogenetic group, by sequencing the 16S ribosomal RNA genes from transformable strains NUB3621, K1041, and NRRL1174. Although all of these strains were described in the literature as B. stearothermophilus, only NRRL1174 is closely related to the type strain of this species. Based on its 16S rDNA sequence and physiological data K1041 appeared to belong to the species B. thermodenitrificans, while NUB3621 showed a slightly closer relationship to B. thermoglucosidasius than to B. stearothermophilus. Therefore we conclude that the trait of transformability, though possibly strain-specific, is not limited to a single species of thermophilic Bacillus. Copyright (C) 1999 Federation of European Microbiological Societies.",

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AU - Jackson, Robin A.

AU - Leak, David J.

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N2 - Few strains of thermophilic Bacillus spp are readily transformable with plasmid DNA. Given the considerable phylogenetic and phenotypic diversity amongst thermophilic bacilli, we have examined whether transformability is a trait associated with a particular phylogenetic group, by sequencing the 16S ribosomal RNA genes from transformable strains NUB3621, K1041, and NRRL1174. Although all of these strains were described in the literature as B. stearothermophilus, only NRRL1174 is closely related to the type strain of this species. Based on its 16S rDNA sequence and physiological data K1041 appeared to belong to the species B. thermodenitrificans, while NUB3621 showed a slightly closer relationship to B. thermoglucosidasius than to B. stearothermophilus. Therefore we conclude that the trait of transformability, though possibly strain-specific, is not limited to a single species of thermophilic Bacillus. Copyright (C) 1999 Federation of European Microbiological Societies.

AB - Few strains of thermophilic Bacillus spp are readily transformable with plasmid DNA. Given the considerable phylogenetic and phenotypic diversity amongst thermophilic bacilli, we have examined whether transformability is a trait associated with a particular phylogenetic group, by sequencing the 16S ribosomal RNA genes from transformable strains NUB3621, K1041, and NRRL1174. Although all of these strains were described in the literature as B. stearothermophilus, only NRRL1174 is closely related to the type strain of this species. Based on its 16S rDNA sequence and physiological data K1041 appeared to belong to the species B. thermodenitrificans, while NUB3621 showed a slightly closer relationship to B. thermoglucosidasius than to B. stearothermophilus. Therefore we conclude that the trait of transformability, though possibly strain-specific, is not limited to a single species of thermophilic Bacillus. Copyright (C) 1999 Federation of European Microbiological Societies.

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Phylogenetic analysis of transformable strains of thermophilic Bacillus species

Abstract

Keywords

ASJC Scopus subject areas

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