Photopolymerization of polydiacetylene in hybrid liposomes: Effect of polymerization on stability and response to pathogenic bacterial toxins

N.T. Thet, W.D. Jamieson, M. Laabei, J.D. Mercer-Chalmers, A.T.A. Jenkins

Research output: Contribution to journalArticle

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Abstract

Liposomes containing lipids and polydiacetylene (PDA) are hybrid systems encompassing both a fluid phospholipid membrane and a polymer scaffold (PDA). However, the biophysical role of PDA in such liposomes is not well understood. In this report, we studied the effects of photopolymerization of PDA on the stability of lipid-PDA liposomes, and their sensitivity to selected purified toxins and bacterial supernatants, using a fluorescence assay. Of the three different types of liposomes with variable lipid chain lengths that were chosen, the degree of polymerization had a significant impact on the long-term stability, and response, to external microbial exotoxins secreted by pathogenic bacteria, namely, Staphylococcus aureus and Pseudomonas aeruginosa. The degree of polymerization of TCDA played an important role in lipid-chain-length- dependent stabilization of lipid-PDA liposomes, as well as in their response to bacterial toxins of S. aureus and P. aeruginosa.
LanguageEnglish
Pages5418-5427
Number of pages10
JournalJournal of Physical Chemistry B
Volume118
Issue number20
Early online date7 May 2014
DOIs
StatusPublished - 22 May 2014

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Photopolymerization
Liposomes
Lipids
Polymerization
Chain length
Phospholipids
Hybrid systems
Scaffolds
Assays
Bacteria
Stabilization
Fluorescence
Membranes
Fluids
Polymers

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Photopolymerization of polydiacetylene in hybrid liposomes : Effect of polymerization on stability and response to pathogenic bacterial toxins. / Thet, N.T.; Jamieson, W.D.; Laabei, M.; Mercer-Chalmers, J.D.; Jenkins, A.T.A.

In: Journal of Physical Chemistry B, Vol. 118, No. 20, 22.05.2014, p. 5418-5427.

Research output: Contribution to journalArticle

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