Photoinduced bidirectional switching in lipid membranes containing azobenzene glycolipids

Jonas E. Warias; Franziska Reise; Svenja C. Hövelmann; Rajendra P. Giri; Michael Röhrl; Jule Kuhn; Malte Jacobsen; Kuntal Chatterjee; Thomas Arnold; Chen Shen; Sven Festersen; Andrea Sartori; Philipp Jordt; Olaf M. Magnussen; Thisbe K. Lindhorst; Bridget M. Murphy

doi:10.1038/s41598-023-38336-x

Photoinduced bidirectional switching in lipid membranes containing azobenzene glycolipids

Jonas E. Warias, Franziska Reise, Svenja C. Hövelmann, Rajendra P. Giri, Michael Röhrl, Jule Kuhn, Malte Jacobsen, Kuntal Chatterjee, Thomas Arnold, Chen Shen, Sven Festersen, Andrea Sartori, Philipp Jordt, Olaf M. Magnussen, Thisbe K. Lindhorst, Bridget M. Murphy

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5 Citations (SciVal)

Abstract

Following the reaction of biological membranes to external stimuli reveals fundamental insights into cellular function. Here, self-assembled lipid monolayers act as model membranes containing photoswitchable azobenzene glycolipids for investigating structural response during isomerization by combining Langmuir isotherms with X-ray scattering. Controlled in-situ trans/cis photoswitching of the azobenzene N = N double bond alters the DPPC monolayer structure, causing reproducible changes in surface pressure and layer thickness, indicating monolayer reorientation. Interestingly, for monolayers containing azobenzene glycolipids, along with the expected DPPC phase transitions an additional discontinuity is observed. The associated reorintation represents a crossover point, with the surface pressure and layer thickness changing in opposite directions above and below. This is evidence that the azobenzene glycolipids themselves change orientation within the monolayer. Such behaviour suggests that azobenzene glycolipids can act as a bidirectional switch in DPPC monolayers providing a tool to investigate membrane structure–function relationships in depth.

Original language	English
Article number	11480
Journal	Scientific Reports
Volume	13
Issue number	1
Early online date	16 Jul 2023
DOIs	https://doi.org/10.1038/s41598-023-38336-x
Publication status	Published - 31 Dec 2023
Externally published	Yes

Bibliographical note

Funding Information:
We acknowledge DESY (Hamburg, Germany), a member of the Helmholtz Association HGF, for the provision of experimental facilities. Parts of this research were carried out at PETRA III and we would like to thank Florian Bertram and Rene Kirchhof for assistance in using P08 and Milena Lippmann for her assistance in using the PETRA III chemistry laboratory. Beamtime was allocated for proposals I-20180411 and I-20160864. We acknowledge Diamond Light Source for time on Beamline I07 under Proposal [SI15284-1] The research was supported by the SFB 677, BMBF 05K19FK2 and the early science program of the center for molecular water science, DESY.

Funding Information:
We acknowledge DESY (Hamburg, Germany), a member of the Helmholtz Association HGF, for the provision of experimental facilities. Parts of this research were carried out at PETRA III and we would like to thank Florian Bertram and Rene Kirchhof for assistance in using P08 and Milena Lippmann for her assistance in using the PETRA III chemistry laboratory. Beamtime was allocated for proposals I-20180411 and I-20160864. We acknowledge Diamond Light Source for time on Beamline I07 under Proposal [SI15284-1] The research was supported by the SFB 677, BMBF 05K19FK2 and the early science program of the center for molecular water science, DESY.

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Cite this

Warias, J. E., Reise, F., Hövelmann, S. C., Giri, R. P., Röhrl, M., Kuhn, J., Jacobsen, M., Chatterjee, K., Arnold, T., Shen, C., Festersen, S., Sartori, A., Jordt, P., Magnussen, O. M., Lindhorst, T. K., & Murphy, B. M. (2023). Photoinduced bidirectional switching in lipid membranes containing azobenzene glycolipids. Scientific Reports, 13(1), Article 11480. https://doi.org/10.1038/s41598-023-38336-x

Warias, JE, Reise, F, Hövelmann, SC, Giri, RP, Röhrl, M, Kuhn, J, Jacobsen, M, Chatterjee, K, Arnold, T, Shen, C, Festersen, S, Sartori, A, Jordt, P, Magnussen, OM, Lindhorst, TK & Murphy, BM 2023, 'Photoinduced bidirectional switching in lipid membranes containing azobenzene glycolipids', Scientific Reports, vol. 13, no. 1, 11480. https://doi.org/10.1038/s41598-023-38336-x

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title = "Photoinduced bidirectional switching in lipid membranes containing azobenzene glycolipids",

abstract = "Following the reaction of biological membranes to external stimuli reveals fundamental insights into cellular function. Here, self-assembled lipid monolayers act as model membranes containing photoswitchable azobenzene glycolipids for investigating structural response during isomerization by combining Langmuir isotherms with X-ray scattering. Controlled in-situ trans/cis photoswitching of the azobenzene N = N double bond alters the DPPC monolayer structure, causing reproducible changes in surface pressure and layer thickness, indicating monolayer reorientation. Interestingly, for monolayers containing azobenzene glycolipids, along with the expected DPPC phase transitions an additional discontinuity is observed. The associated reorintation represents a crossover point, with the surface pressure and layer thickness changing in opposite directions above and below. This is evidence that the azobenzene glycolipids themselves change orientation within the monolayer. Such behaviour suggests that azobenzene glycolipids can act as a bidirectional switch in DPPC monolayers providing a tool to investigate membrane structure–function relationships in depth.",

author = "Warias, {Jonas E.} and Franziska Reise and H{\"o}velmann, {Svenja C.} and Giri, {Rajendra P.} and Michael R{\"o}hrl and Jule Kuhn and Malte Jacobsen and Kuntal Chatterjee and Thomas Arnold and Chen Shen and Sven Festersen and Andrea Sartori and Philipp Jordt and Magnussen, {Olaf M.} and Lindhorst, {Thisbe K.} and Murphy, {Bridget M.}",

note = "Funding Information: We acknowledge DESY (Hamburg, Germany), a member of the Helmholtz Association HGF, for the provision of experimental facilities. Parts of this research were carried out at PETRA III and we would like to thank Florian Bertram and Rene Kirchhof for assistance in using P08 and Milena Lippmann for her assistance in using the PETRA III chemistry laboratory. Beamtime was allocated for proposals I-20180411 and I-20160864. We acknowledge Diamond Light Source for time on Beamline I07 under Proposal [SI15284-1] The research was supported by the SFB 677, BMBF 05K19FK2 and the early science program of the center for molecular water science, DESY. Funding Information: We acknowledge DESY (Hamburg, Germany), a member of the Helmholtz Association HGF, for the provision of experimental facilities. Parts of this research were carried out at PETRA III and we would like to thank Florian Bertram and Rene Kirchhof for assistance in using P08 and Milena Lippmann for her assistance in using the PETRA III chemistry laboratory. Beamtime was allocated for proposals I-20180411 and I-20160864. We acknowledge Diamond Light Source for time on Beamline I07 under Proposal [SI15284-1] The research was supported by the SFB 677, BMBF 05K19FK2 and the early science program of the center for molecular water science, DESY. ",

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day = "31",

doi = "10.1038/s41598-023-38336-x",

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T1 - Photoinduced bidirectional switching in lipid membranes containing azobenzene glycolipids

AU - Warias, Jonas E.

AU - Reise, Franziska

AU - Hövelmann, Svenja C.

AU - Giri, Rajendra P.

AU - Röhrl, Michael

AU - Kuhn, Jule

AU - Jacobsen, Malte

AU - Chatterjee, Kuntal

AU - Arnold, Thomas

AU - Shen, Chen

AU - Festersen, Sven

AU - Sartori, Andrea

AU - Jordt, Philipp

AU - Magnussen, Olaf M.

AU - Lindhorst, Thisbe K.

AU - Murphy, Bridget M.

N1 - Funding Information: We acknowledge DESY (Hamburg, Germany), a member of the Helmholtz Association HGF, for the provision of experimental facilities. Parts of this research were carried out at PETRA III and we would like to thank Florian Bertram and Rene Kirchhof for assistance in using P08 and Milena Lippmann for her assistance in using the PETRA III chemistry laboratory. Beamtime was allocated for proposals I-20180411 and I-20160864. We acknowledge Diamond Light Source for time on Beamline I07 under Proposal [SI15284-1] The research was supported by the SFB 677, BMBF 05K19FK2 and the early science program of the center for molecular water science, DESY. Funding Information: We acknowledge DESY (Hamburg, Germany), a member of the Helmholtz Association HGF, for the provision of experimental facilities. Parts of this research were carried out at PETRA III and we would like to thank Florian Bertram and Rene Kirchhof for assistance in using P08 and Milena Lippmann for her assistance in using the PETRA III chemistry laboratory. Beamtime was allocated for proposals I-20180411 and I-20160864. We acknowledge Diamond Light Source for time on Beamline I07 under Proposal [SI15284-1] The research was supported by the SFB 677, BMBF 05K19FK2 and the early science program of the center for molecular water science, DESY.

PY - 2023/12/31

Y1 - 2023/12/31

N2 - Following the reaction of biological membranes to external stimuli reveals fundamental insights into cellular function. Here, self-assembled lipid monolayers act as model membranes containing photoswitchable azobenzene glycolipids for investigating structural response during isomerization by combining Langmuir isotherms with X-ray scattering. Controlled in-situ trans/cis photoswitching of the azobenzene N = N double bond alters the DPPC monolayer structure, causing reproducible changes in surface pressure and layer thickness, indicating monolayer reorientation. Interestingly, for monolayers containing azobenzene glycolipids, along with the expected DPPC phase transitions an additional discontinuity is observed. The associated reorintation represents a crossover point, with the surface pressure and layer thickness changing in opposite directions above and below. This is evidence that the azobenzene glycolipids themselves change orientation within the monolayer. Such behaviour suggests that azobenzene glycolipids can act as a bidirectional switch in DPPC monolayers providing a tool to investigate membrane structure–function relationships in depth.

AB - Following the reaction of biological membranes to external stimuli reveals fundamental insights into cellular function. Here, self-assembled lipid monolayers act as model membranes containing photoswitchable azobenzene glycolipids for investigating structural response during isomerization by combining Langmuir isotherms with X-ray scattering. Controlled in-situ trans/cis photoswitching of the azobenzene N = N double bond alters the DPPC monolayer structure, causing reproducible changes in surface pressure and layer thickness, indicating monolayer reorientation. Interestingly, for monolayers containing azobenzene glycolipids, along with the expected DPPC phase transitions an additional discontinuity is observed. The associated reorintation represents a crossover point, with the surface pressure and layer thickness changing in opposite directions above and below. This is evidence that the azobenzene glycolipids themselves change orientation within the monolayer. Such behaviour suggests that azobenzene glycolipids can act as a bidirectional switch in DPPC monolayers providing a tool to investigate membrane structure–function relationships in depth.

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Photoinduced bidirectional switching in lipid membranes containing azobenzene glycolipids

Abstract

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