Peptides and Anti-peptide Antibodies for Small- and Medium-Scale Peptide and Anti-peptide Affinity Microarrays

Fan Zhang, Sophie Cleaver, Matthew Gardner, Andrea Briones, Mikhail Soloviev

Research output: Chapter or section in a book/report/conference proceedingChapter or section


This chapter describes the principles for selection of antigenic peptides for the development of anti-peptide antibodies suitable for microarray-based multiplex affinity assays and optional mass spectrometry detection. The methods described here are mostly applicable to small- and medium-scale multiplex affinity assay and microarrays. Although the same principles of peptide selection may also be applied to larger-scale arrays (with 100+ features), informatics software and printing methods may well differ. Due to the sheer number of proteins/peptides to be processed and analyzed, dedicated software with high processing capacity and enterprise-level array robotics may be required for larger-scale efforts. This report aims to provide practical advice to those seeking to develop or use arrays with up to ~100 different peptide or protein features.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsM. Cretich, A. Gori
Place of PublicationNew York, U. S. A.
PublisherHumana Press
ISBN (Electronic)9781071627327
ISBN (Print)9781071627310
Publication statusPublished - 25 Sept 2022

Publication series

NameMethods in Molecular Biology

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