@inbook{b3fe11222c6a41b5949106d8823cb503,
title = "Peptides and Anti-peptide Antibodies for Small- and Medium-Scale Peptide and Anti-peptide Affinity Microarrays",
abstract = "This chapter describes the principles for selection of antigenic peptides for the development of anti-peptide antibodies suitable for microarray-based multiplex affinity assays and optional mass spectrometry detection. The methods described here are mostly applicable to small- and medium-scale multiplex affinity assay and microarrays. Although the same principles of peptide selection may also be applied to larger-scale arrays (with 100+ features), informatics software and printing methods may well differ. Due to the sheer number of proteins/peptides to be processed and analyzed, dedicated software with high processing capacity and enterprise-level array robotics may be required for larger-scale efforts. This report aims to provide practical advice to those seeking to develop or use arrays with up to ~100 different peptide or protein features.",
author = "Fan Zhang and Sophie Cleaver and Matthew Gardner and Andrea Briones and Mikhail Soloviev",
year = "2022",
month = sep,
day = "25",
doi = "10.1007/978-1-0716-2732-7_8",
language = "English",
isbn = "9781071627310",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "103--120",
editor = "M. Cretich and A. Gori",
booktitle = "Methods in Molecular Biology",
address = "USA United States",
}