Peptides and Anti-peptide Antibodies for Small- and Medium-Scale Peptide and Anti-peptide Affinity Microarrays

Fan Zhang, Sophie Cleaver, Matthew Gardner, Andrea Briones, Mikhail Soloviev

Research output: Chapter or section in a book/report/conference proceedingChapter or section

Abstract

This chapter describes the principles for selection of antigenic peptides for the development of anti-peptide antibodies suitable for microarray-based multiplex affinity assays and optional mass spectrometry detection. The methods described here are mostly applicable to small- and medium-scale multiplex affinity assay and microarrays. Although the same principles of peptide selection may also be applied to larger-scale arrays (with 100+ features), informatics software and printing methods may well differ. Due to the sheer number of proteins/peptides to be processed and analyzed, dedicated software with high processing capacity and enterprise-level array robotics may be required for larger-scale efforts. This report aims to provide practical advice to those seeking to develop or use arrays with up to ~100 different peptide or protein features.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsM. Cretich, A. Gori
Place of PublicationNew York, U. S. A.
PublisherHumana Press
Pages103-120
ISBN (Electronic)9781071627327
ISBN (Print)9781071627310
DOIs
Publication statusPublished - 25 Sept 2022

Publication series

NameMethods in Molecular Biology
Volume2578

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