PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)

E G Brede; Peter Long; S Zefania; M Rabenandrasana; Tamas Szekely; M Bruford

doi:10.1007/s12686-010-9189-2

PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)

E G Brede, Peter Long, S Zefania, M Rabenandrasana, Tamas Szekely, M Bruford

Research output: Contribution to journal › Article › peer-review

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Abstract

We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species' range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean H-e = 0.544 (range 0.0678-0.885), mean H-o = 0.430 (range 0.0400-0.767) and mean N-a = 4.59 (range 2-10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.

Original language	English
Pages (from-to)	273-277
Number of pages	5
Journal	Conservation Genetics Resources
Volume	2
DOIs	https://doi.org/10.1007/s12686-010-9189-2
Publication status	Published - Sept 2010

Keywords

microsatellite
waterbird
rail
endangered
primer
Madagascar
Amaurornis olivieri Canirallus kioloides

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10.1007/s12686-010-9189-2

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title = "PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)",

abstract = "We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species' range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean H-e = 0.544 (range 0.0678-0.885), mean H-o = 0.430 (range 0.0400-0.767) and mean N-a = 4.59 (range 2-10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.",

keywords = "microsatellite, waterbird, rail, endangered, primer, Madagascar, Amaurornis olivieri Canirallus kioloides",

author = "Brede, {E G} and Peter Long and S Zefania and M Rabenandrasana and Tamas Szekely and M Bruford",

year = "2010",

month = sep,

doi = "10.1007/s12686-010-9189-2",

language = "English",

volume = "2",

pages = "273--277",

journal = "Conservation Genetics Resources",

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TY - JOUR

T1 - PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)

AU - Brede, E G

AU - Long, Peter

AU - Zefania, S

AU - Rabenandrasana, M

AU - Szekely, Tamas

AU - Bruford, M

PY - 2010/9

Y1 - 2010/9

N2 - We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species' range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean H-e = 0.544 (range 0.0678-0.885), mean H-o = 0.430 (range 0.0400-0.767) and mean N-a = 4.59 (range 2-10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.

AB - We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species' range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean H-e = 0.544 (range 0.0678-0.885), mean H-o = 0.430 (range 0.0400-0.767) and mean N-a = 4.59 (range 2-10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.

KW - microsatellite

KW - waterbird

KW - rail

KW - endangered

KW - primer

KW - Madagascar

KW - Amaurornis olivieri Canirallus kioloides

UR - http://www.scopus.com/inward/record.url?scp=84859945440&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1007/s12686-010-9189-2

U2 - 10.1007/s12686-010-9189-2

DO - 10.1007/s12686-010-9189-2

M3 - Article

SN - 1877-7252

VL - 2

SP - 273

EP - 277

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

ER -

PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)

Abstract

Keywords

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