PCP-B class pollen coat proteins are key regulators of the hydration checkpoint in Arabidopsis thaliana pollen-stigma interactions

Ludi Wang, Lisa A. Clarke, Russell J. Eason, Christopher C. Parker, Baoxiu Qi, Rod J. Scott, James Doughty

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Summary

The establishment of pollen-pistil compatibility is strictly regulated by factors derived from both male and female reproductive structures. Highly diverse small cysteine-rich proteins (CRPs) have been found to play multiple roles in plant reproduction, including the earliest stages of the pollen-stigma interaction. Secreted CRPs found in the pollen coat of members of the Brassicaceae, the pollen coat proteins (PCPs), are emerging as important signalling molecules that regulate the pollen-stigma interaction.

Using a combination of protein characterisation, expression, and phylogenetic analyses we identified a novel class of Arabidopsis thaliana pollen-borne CRPs, the PCP-Bs (for pollen coat protein B-class) that are related to embryo surrounding factor (ESF1) developmental regulators. Single and multiple PCP-B mutant lines were utilised in bioassays to assess effects on pollen hydration, adhesion and pollen tube growth.

Our results reveal that pollen hydration is severely impaired when multiple PCP-Bs are lost from then pollen coat. The hydration defect also resulted in reduced pollen adhesion and delayed pollen tube growth in all mutants studied.

These results demonstrate that AtPCP-Bs are key regulators of the hydration ‘checkpoint’ in establishment of pollen-stigma compatibility. In addition we propose that interspecies diversity of PCP-Bs may contribute to reproductive barriers in the Brassicaceae.
LanguageEnglish
Pages764-777
Number of pages14
JournalNew Phytologist
Volume213
Issue number2
Early online date6 Sep 2016
DOIs
StatusPublished - Jan 2017

Fingerprint

Capsid Proteins
Pollen
stigma
Arabidopsis
Arabidopsis thaliana
pollen
cysteine
pollen tubes
Brassicaceae
adhesion
Pollen Tube
Cysteine
pollen proteins
IgA receptor
mutants
plant reproduction
proteins
pistil
Proteins
bioassays

Keywords

  • pollen-stigma interaction, compatibility, pollen coat proteins, pollen hydration, signalling, reproduction, Arabidopsis thaliana

Cite this

PCP-B class pollen coat proteins are key regulators of the hydration checkpoint in Arabidopsis thaliana pollen-stigma interactions. / Wang, Ludi; Clarke, Lisa A.; Eason, Russell J.; Parker, Christopher C.; Qi, Baoxiu; Scott, Rod J.; Doughty, James.

In: New Phytologist, Vol. 213, No. 2, 01.2017, p. 764-777.

Research output: Contribution to journalArticle

Wang, Ludi ; Clarke, Lisa A. ; Eason, Russell J. ; Parker, Christopher C. ; Qi, Baoxiu ; Scott, Rod J. ; Doughty, James. / PCP-B class pollen coat proteins are key regulators of the hydration checkpoint in Arabidopsis thaliana pollen-stigma interactions. In: New Phytologist. 2017 ; Vol. 213, No. 2. pp. 764-777.
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