Patterns of integrin expression in a human mandibular explant model of osteoblast differentiation

J. H. Bennett, D. H. Carter, A. L. Alavi, J. N. Beresford, S. Walsh

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Cell-matrix interaction is crucial in regulating osteoblast differentiation and function. These interactions are themselves regulated, at least in part, by integrins. Although there are some data from mammalian models, few studies have compared integrin expression at different stages of the osteoblast lineage. Here, primary human mandibular osteoblast cultures were grown in the presence of epidermal growth factor (EGF), giving a proliferative, less differentiated phenotype, or of vitamin D-3 and hydrocortisone (D + He), giving a more differentiated phenotype. These cultures were compared with those of cells prepared in the absence of EGF or D + He by fluorescence-activated cell sorter using a panel of monoclonal antibodies to specific integrin heterodimers. To provide in vivo correlation, the same panel of antibodies was used to stain fresh-frozen, undemineralised sections of human mandibular bone. Under baseline conditions the alpha (3), alpha (5), alpha (v) alpha (v)beta (3), beta (3) and beta (1) integrin subunits were expressed strongly by the cells, with low-level expression of the alpha (1), alpha (2) and alpha (4) subunits. In the presence of EGF there was increased a, expression. With D + He, alpha (3) and alpha (5) expression was elevated. Immunohistochemical analysis demonstrated alpha (2), alpha (3), alpha (5), alpha (v)beta (3), beta (1) and beta (3) subunits in cells of the osteoblast lineage; alpha (2) staining was restricted to cells close to the bone surface whilst alpha (v)beta (3) and beta (3) were most frequently localised in the osteocytes. The results proc ide evidence that cells at successive stages of the osteoblast lineage show different patterns of integrin expression. These integrins may be important in cell-matrix interactions leading to osteoblast differentiation.
Original languageEnglish
Pages (from-to)229-238
Number of pages10
JournalArchives of Oral Biology
Volume46
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Osteoblasts
Integrins
Epidermal Growth Factor
Cell Communication
Integrin beta Chains
Integrin beta3
Phenotype
Bone and Bones
Osteocytes
Cholecalciferol
Frozen Sections
Cell Lineage
Hydrocortisone
Coloring Agents
Fluorescence
Monoclonal Antibodies
Staining and Labeling
Antibodies

Cite this

Patterns of integrin expression in a human mandibular explant model of osteoblast differentiation. / Bennett, J. H.; Carter, D. H.; Alavi, A. L.; Beresford, J. N.; Walsh, S.

In: Archives of Oral Biology, Vol. 46, No. 3, 2001, p. 229-238.

Research output: Contribution to journalArticle

Bennett, J. H. ; Carter, D. H. ; Alavi, A. L. ; Beresford, J. N. ; Walsh, S. / Patterns of integrin expression in a human mandibular explant model of osteoblast differentiation. In: Archives of Oral Biology. 2001 ; Vol. 46, No. 3. pp. 229-238.
@article{9a0a4431f184419392be63cca5f82d72,
title = "Patterns of integrin expression in a human mandibular explant model of osteoblast differentiation",
abstract = "Cell-matrix interaction is crucial in regulating osteoblast differentiation and function. These interactions are themselves regulated, at least in part, by integrins. Although there are some data from mammalian models, few studies have compared integrin expression at different stages of the osteoblast lineage. Here, primary human mandibular osteoblast cultures were grown in the presence of epidermal growth factor (EGF), giving a proliferative, less differentiated phenotype, or of vitamin D-3 and hydrocortisone (D + He), giving a more differentiated phenotype. These cultures were compared with those of cells prepared in the absence of EGF or D + He by fluorescence-activated cell sorter using a panel of monoclonal antibodies to specific integrin heterodimers. To provide in vivo correlation, the same panel of antibodies was used to stain fresh-frozen, undemineralised sections of human mandibular bone. Under baseline conditions the alpha (3), alpha (5), alpha (v) alpha (v)beta (3), beta (3) and beta (1) integrin subunits were expressed strongly by the cells, with low-level expression of the alpha (1), alpha (2) and alpha (4) subunits. In the presence of EGF there was increased a, expression. With D + He, alpha (3) and alpha (5) expression was elevated. Immunohistochemical analysis demonstrated alpha (2), alpha (3), alpha (5), alpha (v)beta (3), beta (1) and beta (3) subunits in cells of the osteoblast lineage; alpha (2) staining was restricted to cells close to the bone surface whilst alpha (v)beta (3) and beta (3) were most frequently localised in the osteocytes. The results proc ide evidence that cells at successive stages of the osteoblast lineage show different patterns of integrin expression. These integrins may be important in cell-matrix interactions leading to osteoblast differentiation.",
author = "Bennett, {J. H.} and Carter, {D. H.} and Alavi, {A. L.} and Beresford, {J. N.} and S. Walsh",
note = "ID number: ISI:000167024900006",
year = "2001",
doi = "10.1016/S0003-9969(00)00114-X",
language = "English",
volume = "46",
pages = "229--238",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Patterns of integrin expression in a human mandibular explant model of osteoblast differentiation

AU - Bennett, J. H.

AU - Carter, D. H.

AU - Alavi, A. L.

AU - Beresford, J. N.

AU - Walsh, S.

N1 - ID number: ISI:000167024900006

PY - 2001

Y1 - 2001

N2 - Cell-matrix interaction is crucial in regulating osteoblast differentiation and function. These interactions are themselves regulated, at least in part, by integrins. Although there are some data from mammalian models, few studies have compared integrin expression at different stages of the osteoblast lineage. Here, primary human mandibular osteoblast cultures were grown in the presence of epidermal growth factor (EGF), giving a proliferative, less differentiated phenotype, or of vitamin D-3 and hydrocortisone (D + He), giving a more differentiated phenotype. These cultures were compared with those of cells prepared in the absence of EGF or D + He by fluorescence-activated cell sorter using a panel of monoclonal antibodies to specific integrin heterodimers. To provide in vivo correlation, the same panel of antibodies was used to stain fresh-frozen, undemineralised sections of human mandibular bone. Under baseline conditions the alpha (3), alpha (5), alpha (v) alpha (v)beta (3), beta (3) and beta (1) integrin subunits were expressed strongly by the cells, with low-level expression of the alpha (1), alpha (2) and alpha (4) subunits. In the presence of EGF there was increased a, expression. With D + He, alpha (3) and alpha (5) expression was elevated. Immunohistochemical analysis demonstrated alpha (2), alpha (3), alpha (5), alpha (v)beta (3), beta (1) and beta (3) subunits in cells of the osteoblast lineage; alpha (2) staining was restricted to cells close to the bone surface whilst alpha (v)beta (3) and beta (3) were most frequently localised in the osteocytes. The results proc ide evidence that cells at successive stages of the osteoblast lineage show different patterns of integrin expression. These integrins may be important in cell-matrix interactions leading to osteoblast differentiation.

AB - Cell-matrix interaction is crucial in regulating osteoblast differentiation and function. These interactions are themselves regulated, at least in part, by integrins. Although there are some data from mammalian models, few studies have compared integrin expression at different stages of the osteoblast lineage. Here, primary human mandibular osteoblast cultures were grown in the presence of epidermal growth factor (EGF), giving a proliferative, less differentiated phenotype, or of vitamin D-3 and hydrocortisone (D + He), giving a more differentiated phenotype. These cultures were compared with those of cells prepared in the absence of EGF or D + He by fluorescence-activated cell sorter using a panel of monoclonal antibodies to specific integrin heterodimers. To provide in vivo correlation, the same panel of antibodies was used to stain fresh-frozen, undemineralised sections of human mandibular bone. Under baseline conditions the alpha (3), alpha (5), alpha (v) alpha (v)beta (3), beta (3) and beta (1) integrin subunits were expressed strongly by the cells, with low-level expression of the alpha (1), alpha (2) and alpha (4) subunits. In the presence of EGF there was increased a, expression. With D + He, alpha (3) and alpha (5) expression was elevated. Immunohistochemical analysis demonstrated alpha (2), alpha (3), alpha (5), alpha (v)beta (3), beta (1) and beta (3) subunits in cells of the osteoblast lineage; alpha (2) staining was restricted to cells close to the bone surface whilst alpha (v)beta (3) and beta (3) were most frequently localised in the osteocytes. The results proc ide evidence that cells at successive stages of the osteoblast lineage show different patterns of integrin expression. These integrins may be important in cell-matrix interactions leading to osteoblast differentiation.

UR - http://dx.doi.org/10.1016/S0003-9969(00)00114-X

U2 - 10.1016/S0003-9969(00)00114-X

DO - 10.1016/S0003-9969(00)00114-X

M3 - Article

VL - 46

SP - 229

EP - 238

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 3

ER -