PARP10 influences the proliferation of colorectal carcinoma cells, a preliminary study

ChengFang Wu, Ming Xiao, Yalan Wang, Michael Threadgill, Ming Li, Yi Tang, Xiao Lin, Lian Yang, QingShu Li, Xian Li

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Abstract

PARP10 is an intracellular mono-ADP ribosyltransferase and recent reports suggest that it regulates proliferation of some cell types. However, its effect on the proliferation of colorectal carcinoma cells has not yet been systematically reported. We explored the influence of PARP10 on the proliferation of several colorectal carcinoma cell types and carried out initial studies on the underlying mechanisms. Inhibition of the enzymatic activity of PARP10 led to significantly decreases in proliferative ability in LoVo cells and CT26 cells in vitro and suppressed growth of CT26 tumours in the subaxilliary region in Balb/c mice in vivo. Cell-cycle arrest accompanied these observations. Expression of the nuclear transfer factor β-catenin and it translocation to the nucleus were also affected and the expression of its associated signal proteins Axin2 and c-Myb were increased and decreased, respectively. We demonstrate that PARP10 promotes proliferation of those colorectal carcinoma cells which express significant levels of PARP10. This promotion is suppressed when the enzymatic activity is inhibited. β-Catenin is likely to be the mediator of the antiproliferative effect.
Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalMolecular Biology
Volume54
Issue number2
DOIs
Publication statusPublished - 31 Mar 2020

Keywords

  • PARP10
  • colorectal carcinoma
  • proliferation
  • β-catenin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology

Cite this

Wu, C., Xiao, M., Wang, Y., Threadgill, M., Li, M., Tang, Y., Lin, X., Yang, L., Li, Q., & Li, X. (2020). PARP10 influences the proliferation of colorectal carcinoma cells, a preliminary study. Molecular Biology, 54(2), 220-228. https://doi.org/10.1134/S0026893320020181