PA6 Stromal Cell Co-Culture Enhances SH-SY5Y and VSC4.1 Neuroblastoma Differentiation to Mature Phenotypes

Ross Ferguson, Vasanta Subramanian

Research output: Contribution to journalArticle

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Abstract

Neuroblastoma cell lines such as SH-SY5Y have been used for modelling neurodegenerative diseases and for studying basic mechanisms in neuroscience. Since neuroblastoma cells proliferate and generally do not express markers of mature or functional neurons, we exploited a co-culture system with the stromal cell line PA6 to better induce differentiation to a more physiologically relevant status. We found that co-culture of the neuroblastoma cell lines in the presence of neural inducers such retinoic acid was able to generate a high proportion of quiescent neurons with very long neurites expressing differentiation markers. The co-culture system additionally cuts short the time taken to produce a more mature phenotype. We also show the application of this system to study proteins implicated in motor neuron disease.

LanguageEnglish
Article numbere0159051
JournalPLoS ONE
Volume11
Issue number7
DOIs
StatusPublished - 8 Jul 2016

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stromal cells
coculture
Stromal Cells
Coculture Techniques
Neuroblastoma
Neurons
Cell Culture Techniques
Cells
cell lines
Phenotype
Cell culture
phenotype
Cell Line
neurons
Neurodegenerative diseases
Motor Neuron Disease
neurophysiology
neurites
retinoic acid
Differentiation Antigens

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PA6 Stromal Cell Co-Culture Enhances SH-SY5Y and VSC4.1 Neuroblastoma Differentiation to Mature Phenotypes. / Ferguson, Ross; Subramanian, Vasanta.

In: PLoS ONE, Vol. 11, No. 7, e0159051, 08.07.2016.

Research output: Contribution to journalArticle

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