Ozone gas as a benign sterilization treatment for PLGA nanofibre scaffolds

Carolina Fracalossi Rediguieri, Terezinha de Jesus Andreoli Pinto, Nadia Araci Bou-Chacra, Raquel Galante, Gabriel Lima Barros de Araújo, Tatiana do Nascimento Pedrosa, Silvya Stuchi Maria-Engler, Paul A. De Bank, Paul De Bank

Research output: Contribution to journalArticlepeer-review

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The use of electrospun nanofibres for tissue engineering and regenerative medicine applications is a growing trend as they provide improved support for cell proliferation and survival due, in part, to their morphology mimicking that of the extracellular matrix. Sterilization is a critical step in the fabrication process of implantable biomaterial scaffolds for clinical use, but many of the existing methods employed to date can negatively affect scaffold properties and performance. Poly(lactic-co-glycolic acid) (PLGA) has been widely used as a biodegradable polymer for 3D scaffolds, and can be significantly affected by current sterilization techniques. The aim of this study was to investigate pulsed ozone gas as an alternative method for sterilizing PLGA nanofibres. The morphology, mechanical properties, physicochemical properties, and response of cells to PLGA nanofibre scaffolds were assessed following different degrees of ozone gas sterilization. This treatment killed Geobacillus stearothermophilus spores, the most common biological indicator used for validation of sterilization processes. In addition, the method preserved all of the characteristics of non-sterilized PLGA nanofibres at all degrees of sterilization tested. These findings suggest that ozone gas can be applied as an alternative method for sterilizing electrospun PLGA nanofibre scaffolds without detrimental effects.
Original languageEnglish
Pages (from-to)338-347
Number of pages10
JournalTissue Engineering part C Methods
Issue number4
Early online date23 Feb 2016
Publication statusPublished - 1 Apr 2016


  • Polymeric scaffolds
  • Nanofibres
  • Sterilization
  • Tissue engineering
  • Regenerative medicine


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