Organization of the endoplasmic reticulum in dividing cells of the gymnosperms Pinus brutia and Pinus nigra, and of the pterophyte Asplenium nidus

M. Zachariadis, H. Quader, B. Galatis, P. Apostolakos

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23 Citations (SciVal)


Endoplasmic reticulum (ER) organization in the dividing cells of the pterophyte Asplenium nidus and of the gymnosperms Pinus brutia and Pinus nigra has been studied by immunolocalization techniques using the monoclonal antibody 2E7, which recognizes luminar ER resident proteins containing C-terminal HDEL sequences. In the pterophyte, the ER reorganization during cell cycle is similar to that in angiosperms. Among others, prominent ER gatherings were found at the mitotic spindle poles and in the phragmoplast during cytokinesis. However, in the gymnosperms examined, the ER displays a unique pattern of reorganization not described so far. In both the Pinus species, well-defined ER patterns are successively formed during cell cycle. They are the preprophase ER-band, the prophase- metaphase- and anaphase ER-spindle, the interzonal ER-system, the ER-phragmoplast and an ER-system lining the daughter cell wall. The ER patterns are closely similar to that of the correspondent microtubule (MT) arrangements with which they are co-organized. Observations made on P. nigra root-cells affected by oryzalin, colchicine and cytochalasin D favour the conclusion that the pattern of ER organization is controlled during mitosis and cytokinesis by the MT cytoskeleton.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalCell Biology International
Issue number1
Publication statusPublished - 1 Jan 2003

Bibliographical note

Funding Information:
This work was supported by a grant from the Greek-German Technology Program (GRI-045-099). We greatly appreciate the gifting of the antibody against the ER residential proteins by Dr Napier (Horticulture Research International, East Malling, West Mallin, Kent ME19 6BJ, UK) and of the mouse serum by Dr Komis Ch. (Hellenic Pasteur Institute, Athens, Greece). We would also like to thank George Komis for performing the AF localization. The State Scholarship Foundation awarded Michael Zachariadis a scholarship.


  • Asplenium nidus
  • Dividing cells
  • ER-organization
  • Pinus brutia
  • Pinus nigra

ASJC Scopus subject areas

  • Cell Biology


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