Optimizing the optical trapping stiffness of holographically trapped microrods using high-speed video tracking

David B. Phillips, David M. Carberry, S.H. Simpson, Henrik Schäfer, M. Steinhart, R. Bowman, Graham M. Gibson, Miles J. Padgett, S. Hanna, M J Miles

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Dielectric microrods can be trapped horizontally in pairs of holographically controlled optical traps. External forces acting on these microrods are registered via the rotational and translational displacement of the microrod relative to the traps. In the following paper we demonstrate accurate, real-time tracking of this displacement in two dimensions. The precision of the method is estimated and the translational and rotational stiffness coefficients of the trapped microrod are evaluated by analysing the thermal motion and the Stokes drag. The variation of these stiffness coefficients relative to the displacement of the traps from the ends of the microrods is measured, and optimal trapping conditions are located.

Original languageEnglish
Article number044023
JournalJournal of Optics
Volume13
Issue number4
DOIs
Publication statusPublished - Apr 2011

Keywords

  • optical trap calibration
  • optical tweezers
  • particle tracking

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials

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