Optimization of label-free DNA detection with electrochemical impedance spectroscopy using PNA probes

SD Keighley, Pedro Estrela, P Li, P Migliorato

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

DNA biosensors, especially those based upon detection of the intrinsic negative charge of target DNA, can be greatly improved by the use of uncharged peptide nucleic acid (PNA) probes. Hybridization causes an increased electrostatic barrier for the negatively charged ferri/ferrocyanide redox couple, resulting in an increase in charge transfer resistance Rct that is measured using electrochemical impedance spectroscopy. We report on the optimization of PNA probe surface density by the simultaneous co-immobilization of thiol-modified probes and mercaptohexanol, with the PNA surface density controlled by the thiol mole ratio in solution. Maximum Rct change upon hybridization is obtained with 10% PNA mole fraction. The effect of the measurement buffer ionic strength is investigated. The electrostatic barrier for charge transfer to the ferri/ferrocyanide redox couple is approximately independent of ionic strength with PNA probes, but greatly increases with decreasing ionic strength, after hybridization with target DNA. This significantly enhances the Rct change upon hybridization. The optimization of PNA surface density and measurement buffer ionic strength leads to a 385-fold increase in Rct upon hybridization, a factor of 100 larger than previously reported results using either PNA or DNA probes.
Original languageEnglish
Pages (from-to)906-911
Number of pages6
JournalBiosensors and Bioelectronics
Volume24
Issue number4
Early online date4 Aug 2008
DOIs
Publication statusPublished - 1 Dec 2008

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