Optimisation of glycogen quantification in mixed microbial cultures

Ana B. Lanham, Ana R. Ricardo, Marta Coma, Joana Fradinho, Mónica Carvalheira, Adrian Oehmen, Gilda Carvalho, Maria A M Reis

Research output: Contribution to journalArticlepeer-review

59 Citations (SciVal)


This study addressed the key factors affecting the extraction and quantification of glycogen from floccular and granular mixed microbial cultures collected from activated sludge, nutrient removal systems and photosynthetic consortiums: acid concentration, hydrolysis time and concentration of biomass in the hydrolysis. Response surface modelling indicated that 0.9M HCl and a biomass concentration of 1mgmL -1 were optimal conditions for performing acid hydrolysis. Floccular samples only needed a 2-h hydrolysis time whereas granular samples required as much as 5h. An intermediate 3h yielded an error of 10% compared to the results obtained with the hydrolysis times specifically tailored to the type of biomass and can thus be recommended as a practical compromise.

Original languageEnglish
Pages (from-to)518-525
Number of pages8
JournalBioresource Technology
Publication statusPublished - Aug 2012


  • Carbohydrate analysis
  • Design of experiments
  • Floccular and granular sludge
  • Glycogen quantification
  • Response surface modelling


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