Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools

Robert Charles Hider; Charareh Pourzand; Yongmin Ma; Agostino Cilibrizzi

doi:10.3390/molecules28186467

Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools

Robert Charles Hider, Charareh Pourzand, Yongmin Ma, Agostino Cilibrizzi

Research output: Contribution to journal › Review article › peer-review

3 Citations (SciVal)

Abstract

The chemical nature of intracellular labile iron pools (LIPs) is described. By virtue of the kinetic lability of these pools, it is suggested that the isolation of such species by chromatography methods will not be possible, but rather mass spectrometric techniques should be adopted. Iron-sensitive fluorescent probes, which have been developed for the detection and quantification of LIP, are described, including those specifically designed to monitor cytosolic, mitochondrial, and lysosomal LIPs. The potential of near-infrared (NIR) probes for in vivo monitoring of LIP is discussed.

Original language	English
Article number	6467
Number of pages	21
Journal	Molecules
Volume	28
Issue number	18
Early online date	6 Sept 2023
DOIs	https://doi.org/10.3390/molecules28186467
Publication status	Published - 6 Sept 2023

Keywords

fluorescence
imaging
iron sensitive optical probes
labile iron pools
near-infrared probes

ASJC Scopus subject areas

Drug Discovery
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Physical and Theoretical Chemistry
Pharmaceutical Science
Organic Chemistry

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10.3390/molecules28186467Licence: CC BY

Cite this

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title = "Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools",

abstract = "The chemical nature of intracellular labile iron pools (LIPs) is described. By virtue of the kinetic lability of these pools, it is suggested that the isolation of such species by chromatography methods will not be possible, but rather mass spectrometric techniques should be adopted. Iron-sensitive fluorescent probes, which have been developed for the detection and quantification of LIP, are described, including those specifically designed to monitor cytosolic, mitochondrial, and lysosomal LIPs. The potential of near-infrared (NIR) probes for in vivo monitoring of LIP is discussed.",

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author = "Hider, {Robert Charles} and Charareh Pourzand and Yongmin Ma and Agostino Cilibrizzi",

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TY - JOUR

T1 - Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools

AU - Hider, Robert Charles

AU - Pourzand, Charareh

AU - Ma, Yongmin

AU - Cilibrizzi, Agostino

PY - 2023/9/6

Y1 - 2023/9/6

N2 - The chemical nature of intracellular labile iron pools (LIPs) is described. By virtue of the kinetic lability of these pools, it is suggested that the isolation of such species by chromatography methods will not be possible, but rather mass spectrometric techniques should be adopted. Iron-sensitive fluorescent probes, which have been developed for the detection and quantification of LIP, are described, including those specifically designed to monitor cytosolic, mitochondrial, and lysosomal LIPs. The potential of near-infrared (NIR) probes for in vivo monitoring of LIP is discussed.

AB - The chemical nature of intracellular labile iron pools (LIPs) is described. By virtue of the kinetic lability of these pools, it is suggested that the isolation of such species by chromatography methods will not be possible, but rather mass spectrometric techniques should be adopted. Iron-sensitive fluorescent probes, which have been developed for the detection and quantification of LIP, are described, including those specifically designed to monitor cytosolic, mitochondrial, and lysosomal LIPs. The potential of near-infrared (NIR) probes for in vivo monitoring of LIP is discussed.

KW - fluorescence

KW - imaging

KW - iron sensitive optical probes

KW - labile iron pools

KW - near-infrared probes

UR - http://www.scopus.com/inward/record.url?scp=85172775134&partnerID=8YFLogxK

U2 - 10.3390/molecules28186467

DO - 10.3390/molecules28186467

M3 - Review article

SN - 1420-3049

VL - 28

JO - Molecules

JF - Molecules

IS - 18

M1 - 6467

ER -

Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools

Abstract

Keywords

ASJC Scopus subject areas

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