On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1

Marcus Johns, Amanda Bernardes, Eduardo Ribeiro De Azevêdo, Francisco Guimarães, John Lowe, Ella Gale, Igor Polikarpov, Janet Scott, Ram Sharma

Research output: Contribution to journalArticle

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Abstract

Cellulose-based hydrogel materials prepared by regeneration from cellulose solutions in ionic liquids, or ionic liquid containing solvent mixtures (organic electrolyte solutions), are becoming widely used in a range of applications from tissue scaffolds to membrane ionic diodes. In all such applications knowledge of the nature of the hydrogel with regards to porosity (pore size and tortuosity) and material structure and surface properties (crystallinity and hydrophobicity) is critical. Here we report significant changes in hydrogel properties, based on the choice of cellulose raw material (α- or bacterial cellulose – with differing degree of polymerization) and regeneration solvent (methanol or water). Focus is on bioaffinity applications, but the findings have wide ramifications, including in biomedical applications and cellulose saccharification. Specifically, we report that the choice of cellulose and regeneration solvent influences the surface area accessible to a family 1 carbohydrate-binding module (CBM), CBM affinity for the cellulose material, and rate of migration through the hydrogel. By regenerating bacterial cellulose in water, a maximum accessible surface area of 33 m2 g−1 was achieved. However, the highest CBM migration rate, 1.76 μm2 min−1, was attained by regenerating α-cellulose in methanol, which also resulted in the maximum affinity of the biomolecule for the material. Thus, it is clear that if regenerated cellulose hydrogels are to be used as support materials in bioaffinity (or other) applications, a balance between accessible surface area and affinity, or migration rate, must be achieved.
LanguageEnglish
Pages3879-3887
Number of pages9
JournalJournal of Materials Chemistry B
Volume5
Issue number21
DOIs
StatusPublished - 3 May 2017

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Hydrogels
Biomolecules
Carbohydrates
Cellulose
Hydrogel
Ionic Liquids
Ionic liquids
Methanol
Tissue Scaffolds
Saccharification
Water
Hydrophobicity
Electrolytes
Pore size
Surface properties
Raw materials
Diodes
Porosity
Polymerization
Membranes

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On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1. / Johns, Marcus; Bernardes, Amanda; Ribeiro De Azevêdo, Eduardo; Guimarães, Francisco; Lowe, John; Gale, Ella; Polikarpov, Igor; Scott, Janet; Sharma, Ram.

In: Journal of Materials Chemistry B , Vol. 5, No. 21, 03.05.2017, p. 3879-3887.

Research output: Contribution to journalArticle

Johns, M, Bernardes, A, Ribeiro De Azevêdo, E, Guimarães, F, Lowe, J, Gale, E, Polikarpov, I, Scott, J & Sharma, R 2017, 'On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1' Journal of Materials Chemistry B , vol 5, no. 21, pp. 3879-3887. DOI: 10.1039/C7TB00176B
Johns M, Bernardes A, Ribeiro De Azevêdo E, Guimarães F, Lowe J, Gale E et al. On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1. Journal of Materials Chemistry B . 2017 May 3;5(21):3879-3887. Available from, DOI: 10.1039/C7TB00176B
Johns, Marcus ; Bernardes, Amanda ; Ribeiro De Azevêdo, Eduardo ; Guimarães, Francisco ; Lowe, John ; Gale, Ella ; Polikarpov, Igor ; Scott, Janet ; Sharma, Ram. / On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1. In: Journal of Materials Chemistry B . 2017 ; Vol. 5, No. 21. pp. 3879-3887
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