On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1

Marcus Johns, Amanda Bernardes, Eduardo Ribeiro De Azevêdo, Francisco Guimarães, John Lowe, Ella Gale, Igor Polikarpov, Janet Scott, Ram Sharma

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13 Citations (Scopus)

Abstract

Cellulose-based hydrogel materials prepared by regeneration from cellulose solutions in ionic liquids, or ionic liquid containing solvent mixtures (organic electrolyte solutions), are becoming widely used in a range of applications from tissue scaffolds to membrane ionic diodes. In all such applications knowledge of the nature of the hydrogel with regards to porosity (pore size and tortuosity) and material structure and surface properties (crystallinity and hydrophobicity) is critical. Here we report significant changes in hydrogel properties, based on the choice of cellulose raw material (α- or bacterial cellulose – with differing degree of polymerization) and regeneration solvent (methanol or water). Focus is on bioaffinity applications, but the findings have wide ramifications, including in biomedical applications and cellulose saccharification. Specifically, we report that the choice of cellulose and regeneration solvent influences the surface area accessible to a family 1 carbohydrate-binding module (CBM), CBM affinity for the cellulose material, and rate of migration through the hydrogel. By regenerating bacterial cellulose in water, a maximum accessible surface area of 33 m2 g−1 was achieved. However, the highest CBM migration rate, 1.76 μm2 min−1, was attained by regenerating α-cellulose in methanol, which also resulted in the maximum affinity of the biomolecule for the material. Thus, it is clear that if regenerated cellulose hydrogels are to be used as support materials in bioaffinity (or other) applications, a balance between accessible surface area and affinity, or migration rate, must be achieved.
Original languageEnglish
Pages (from-to)3879-3887
Number of pages9
JournalJournal of Materials Chemistry B
Volume5
Issue number21
DOIs
Publication statusPublished - 3 May 2017

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  • Datasets

    Regenerated Cellulose Hydrogels as Bioaffinity Attachment Supports for Carbohydrate-Binding Module 1

    Johns, M. (Creator), Bernardes, A. (Creator), Ribeiro De Azevêdo, E. (Creator), Guimarães, F. (Creator), Lowe, J. (Creator), Gale, E. (Creator), Polikarpov, I. (Creator), Scott, J. L. (Creator) & Sharma, R. (Creator), University of Bath, 2017

    Dataset

    Cite this

    Johns, M., Bernardes, A., Ribeiro De Azevêdo, E., Guimarães, F., Lowe, J., Gale, E., Polikarpov, I., Scott, J., & Sharma, R. (2017). On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1. Journal of Materials Chemistry B , 5(21), 3879-3887. https://doi.org/10.1039/C7TB00176B