On Plasma Activated Acetyl Donors: Comparing the Antibacterial Efficacy of Tetraacetylethylenediamine and Pentaacetate Glucose

Endre J. Szili, Bethany L. Patenall, Adrian Fellows, Dharmit Mistry, A. Toby A. Jenkins, Robert D. Short, Bhagirath Ghimire

Research output: Contribution to journalArticlepeer-review

1 Citation (SciVal)

Abstract

The study compares how acetyl donor molecules tetraacetylethylenediamine (TAED) and pentaacetate glucose (PAG) improve the antibacterial efficacy of solutions activated with a low-temperature atmospheric-pressure argon plasma jet. Plasma activation of solubilised TAED and PAG produce solutions with different chemical compositions and oxidative potentials. Both acetyl donor molecules enhance the hydrogen peroxide (H2O2) concentration in solution with TAED being more effective compared to PAG. However, PAG is more effective at forming peracetic acid (PAA) from reaction of its acetyl donor groups with plasma generated H2O2. The enhanced oxidative potential of plasma activated TAED and PAG solutions were shown to significantly improve bactericidal activity against common wound pathogens Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus compared to plasma activated water produced without acetyl donors. Furthermore, the oxidative capacity of plasma activated PAG was least affected by the bacterial oxidative defence enzyme catalase, attributed to the high concentration of PAA produced in this formulation. Overall, the above data show that acetyl donors may help improve next generation of antimicrobial formulations produced by plasma, which might help combat increasing problems of antimicrobial resistance.

Original languageEnglish
Pages (from-to)423-435
Number of pages13
JournalPlasma
Volume5
Issue number4
Early online date13 Oct 2022
DOIs
Publication statusPublished - 31 Dec 2022

Bibliographical note

Funding Information:
This research was funded by: EPSRC (Grant Nos. EP/R003556/1 and EP/V00607X/1), Australian Research Council Future (Fellowship No. FT190100263), the National Health Medical Research Council Ideas (Grant No. 2002510), and the Future Industries Accelerator Mobility Scheme MOB024.

Keywords

  • antibacterial
  • antimicrobial resistance
  • hydrogen peroxide
  • low-temperature atmospheric-pressure argon plasma jet
  • pentaacetate glucose
  • peracetic acid
  • tetraacetylethylenediamine

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Materials Science (miscellaneous)
  • Physics and Astronomy (miscellaneous)

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