On-line Quantification of Thickness and Strength of Single and Mixed Species Biofilm Grown Under Controlled Laminar Flow Conditions

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Abstract

This study describes cleaning investigations of biofilms comprised of Escherichia coli and Burkholderia cepacia grown on polyethylene, stainless steel and glass substrates. Their adherence behaviour was determined under controlled hydrodynamic conditions using the non-contact technique of Fluid Dynamic Gauging (FDG). FDG utilises flow data to estimate (i) the adhesive (between biofilm and substrate)/cohesive (between cells and extracellular polymeric substances) strengths, and (ii) the thicknesses of biofilms. The thickness of single and mixed species biofilms increased linearly with time and plateaued at 14 days with no significant reduction thereafter. The asymptotic thickness of mixed species biofilm were thinner than E. coli biofilms. The adhesive strength, on the other hand, peaked at approximately 14 days with a significant reduction thereafter. The results showed that the development of biofilm thickness and attachment strength are not affected by the range of surface roughness and surface energy employed. However, the increase in strength is strongly correlated to the protein and glucose content of the biofilms. Confocal laser scanning microscopy results confirmed an increase in the percentage of dead cells after 21 days, contributing to the weakening of the biofilms. Interrupting the flow of media during biofilm development had a negligible impact upon the thickness, but was found to significantly increase the biofilm strength.

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laminar flow
Biofilms
strength (mechanics)
Laminar flow
biofilm
Hydrodynamics
Gaging
Fluid dynamics
adhesives
Adhesives
Escherichia coli
fluid mechanics
Burkholderia cepacia
surface roughness
Stainless Steel
confocal laser scanning microscopy
Polyethylene
stainless steel
Substrates
Interfacial energy

Keywords

  • Biofilms
  • Drip-flow
  • Strength
  • Burkholderia cepacia
  • Escherichia coli
  • Thickness

Cite this

@article{210e30b0e53e44e2ab13cffc23506eea,
title = "On-line Quantification of Thickness and Strength of Single and Mixed Species Biofilm Grown Under Controlled Laminar Flow Conditions",
abstract = "This study describes cleaning investigations of biofilms comprised of Escherichia coli and Burkholderia cepacia grown on polyethylene, stainless steel and glass substrates. Their adherence behaviour was determined under controlled hydrodynamic conditions using the non-contact technique of Fluid Dynamic Gauging (FDG). FDG utilises flow data to estimate (i) the adhesive (between biofilm and substrate)/cohesive (between cells and extracellular polymeric substances) strengths, and (ii) the thicknesses of biofilms. The thickness of single and mixed species biofilms increased linearly with time and plateaued at 14 days with no significant reduction thereafter. The asymptotic thickness of mixed species biofilm were thinner than E. coli biofilms. The adhesive strength, on the other hand, peaked at approximately 14 days with a significant reduction thereafter. The results showed that the development of biofilm thickness and attachment strength are not affected by the range of surface roughness and surface energy employed. However, the increase in strength is strongly correlated to the protein and glucose content of the biofilms. Confocal laser scanning microscopy results confirmed an increase in the percentage of dead cells after 21 days, contributing to the weakening of the biofilms. Interrupting the flow of media during biofilm development had a negligible impact upon the thickness, but was found to significantly increase the biofilm strength.",
keywords = "Biofilms, Drip-flow, Strength, Burkholderia cepacia, Escherichia coli, Thickness",
author = "Oliver Peck and Yong-Min Chew and Michael Bird",
year = "2018",
month = "8",
day = "22",
doi = "10.1016/j.fbp.2018.08.009",
language = "English",
journal = "Food and Bioproducts Processing",
issn = "0960-3085",
publisher = "Institution of Chemical Engineers",

}

TY - JOUR

T1 - On-line Quantification of Thickness and Strength of Single and Mixed Species Biofilm Grown Under Controlled Laminar Flow Conditions

AU - Peck, Oliver

AU - Chew, Yong-Min

AU - Bird, Michael

PY - 2018/8/22

Y1 - 2018/8/22

N2 - This study describes cleaning investigations of biofilms comprised of Escherichia coli and Burkholderia cepacia grown on polyethylene, stainless steel and glass substrates. Their adherence behaviour was determined under controlled hydrodynamic conditions using the non-contact technique of Fluid Dynamic Gauging (FDG). FDG utilises flow data to estimate (i) the adhesive (between biofilm and substrate)/cohesive (between cells and extracellular polymeric substances) strengths, and (ii) the thicknesses of biofilms. The thickness of single and mixed species biofilms increased linearly with time and plateaued at 14 days with no significant reduction thereafter. The asymptotic thickness of mixed species biofilm were thinner than E. coli biofilms. The adhesive strength, on the other hand, peaked at approximately 14 days with a significant reduction thereafter. The results showed that the development of biofilm thickness and attachment strength are not affected by the range of surface roughness and surface energy employed. However, the increase in strength is strongly correlated to the protein and glucose content of the biofilms. Confocal laser scanning microscopy results confirmed an increase in the percentage of dead cells after 21 days, contributing to the weakening of the biofilms. Interrupting the flow of media during biofilm development had a negligible impact upon the thickness, but was found to significantly increase the biofilm strength.

AB - This study describes cleaning investigations of biofilms comprised of Escherichia coli and Burkholderia cepacia grown on polyethylene, stainless steel and glass substrates. Their adherence behaviour was determined under controlled hydrodynamic conditions using the non-contact technique of Fluid Dynamic Gauging (FDG). FDG utilises flow data to estimate (i) the adhesive (between biofilm and substrate)/cohesive (between cells and extracellular polymeric substances) strengths, and (ii) the thicknesses of biofilms. The thickness of single and mixed species biofilms increased linearly with time and plateaued at 14 days with no significant reduction thereafter. The asymptotic thickness of mixed species biofilm were thinner than E. coli biofilms. The adhesive strength, on the other hand, peaked at approximately 14 days with a significant reduction thereafter. The results showed that the development of biofilm thickness and attachment strength are not affected by the range of surface roughness and surface energy employed. However, the increase in strength is strongly correlated to the protein and glucose content of the biofilms. Confocal laser scanning microscopy results confirmed an increase in the percentage of dead cells after 21 days, contributing to the weakening of the biofilms. Interrupting the flow of media during biofilm development had a negligible impact upon the thickness, but was found to significantly increase the biofilm strength.

KW - Biofilms

KW - Drip-flow

KW - Strength

KW - Burkholderia cepacia

KW - Escherichia coli

KW - Thickness

UR - http://www.scopus.com/inward/record.url?scp=85052739093&partnerID=8YFLogxK

U2 - 10.1016/j.fbp.2018.08.009

DO - 10.1016/j.fbp.2018.08.009

M3 - Special issue

JO - Food and Bioproducts Processing

T2 - Food and Bioproducts Processing

JF - Food and Bioproducts Processing

SN - 0960-3085

ER -