Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst

Gloryn Chia Le Bin, Silvia Muñoz-Descalzo, Agata Kurowski, Harry Leitch, Xinghua Lou, William Mansfield, Charles Etienne-Dumeau, Nils Grabole, Carla Mulas, Hitoshi Niwa, Anna-katerina Hadjantonakis, Jennifer Nichols

Research output: Contribution to journalArticle

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Abstract

The transcription factor Oct4 is required in vitro for establishment and maintenance of embryonic stem cells and for reprogramming somatic cells to pluripotency. In vivo, it prevents the ectopic differentiation of early embryos into trophoblast. Here, we further explore the role of Oct4 in blastocyst formation and specification of epiblast versus primitive endoderm lineages using conditional genetic deletion. Experiments involving mouse embryos deficient for both maternal and zygotic Oct4 suggest that it is dispensable for zygote formation, early cleavage and activation of Nanog expression. Nanog protein is significantly elevated in the presumptive inner cell mass of Oct4 null embryos, suggesting an unexpected role for Oct4 in attenuating the level of Nanog, which might be significant for priming differentiation during epiblast maturation. Induced deletion of Oct4 during the morula to blastocyst transition disrupts the ability of inner cell mass cells to adopt lineage-specific identity and acquire the molecular profile characteristic of either epiblast or primitive endoderm. Sox17, a marker of primitive endoderm, is not detected following prolonged culture of such embryos, but can be rescued by provision of exogenous FGF4. Interestingly, functional primitive endoderm can be rescued in Oct4-deficient embryos in embryonic stem cell complementation assays, but only if the host embryos are at the preblastocyst stage. We conclude that cell fate decisions within the inner cell mass are dependent upon Oct4 and that Oct4 is not cellautonomously required for the differentiation of primitive endoderm derivatives, as long as an appropriate developmental environment is established.
LanguageEnglish
Pages1001-1010
Number of pages10
JournalDevelopment
Volume141
Issue number5
Early online date6 Feb 2014
DOIs
StatusPublished - 1 Mar 2014

Fingerprint

Blastocyst Inner Cell Mass
Endoderm
Embryonic Structures
Germ Layers
Blastocyst
Embryonic Stem Cells
Colony-Forming Units Assay
Morula
Zygote
Trophoblasts
Transcription Factors
Maintenance
Mothers

Keywords

  • Blastocyst
  • Chimaera
  • Nanog
  • Oct4 (Pou5f1)
  • Primitive endoderm
  • Sox17

Cite this

Le Bin, G. C., Muñoz-Descalzo, S., Kurowski, A., Leitch, H., Lou, X., Mansfield, W., ... Nichols, J. (2014). Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. Development, 141(5), 1001-1010. https://doi.org/10.1242/dev.096875

Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. / Le Bin, Gloryn Chia; Muñoz-Descalzo, Silvia; Kurowski, Agata; Leitch, Harry; Lou, Xinghua; Mansfield, William; Etienne-Dumeau, Charles; Grabole, Nils; Mulas, Carla; Niwa, Hitoshi; Hadjantonakis, Anna-katerina; Nichols, Jennifer.

In: Development, Vol. 141, No. 5, 01.03.2014, p. 1001-1010.

Research output: Contribution to journalArticle

Le Bin, GC, Muñoz-Descalzo, S, Kurowski, A, Leitch, H, Lou, X, Mansfield, W, Etienne-Dumeau, C, Grabole, N, Mulas, C, Niwa, H, Hadjantonakis, A & Nichols, J 2014, 'Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst', Development, vol. 141, no. 5, pp. 1001-1010. https://doi.org/10.1242/dev.096875
Le Bin GC, Muñoz-Descalzo S, Kurowski A, Leitch H, Lou X, Mansfield W et al. Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. Development. 2014 Mar 1;141(5):1001-1010. https://doi.org/10.1242/dev.096875
Le Bin, Gloryn Chia ; Muñoz-Descalzo, Silvia ; Kurowski, Agata ; Leitch, Harry ; Lou, Xinghua ; Mansfield, William ; Etienne-Dumeau, Charles ; Grabole, Nils ; Mulas, Carla ; Niwa, Hitoshi ; Hadjantonakis, Anna-katerina ; Nichols, Jennifer. / Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. In: Development. 2014 ; Vol. 141, No. 5. pp. 1001-1010.
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