Nuclear envelope formation: Mind the gaps

During mitosis in metazoans, the nuclear envelope (NE) breaks down at prophase and reassembles at telophase. The regulation of NE assembly is essential to correct cell functioning. The complex issue of the regulation of NE formation remains to be solved. It is still uncertain that a single mechanism depictsNEformation during mitosis. The aim of this review is to address some of the cytological, biophysical, and molecular aspects of models ofNEformation. Our emphasis is on the role of lipids and their modifying enzymes in envelope assembly. We consider how the NE can be used as a model in characterizing membrane dynamics during membrane fusion. Fusion mechanisms that give insight into the formation of the double membrane of the envelope are summarized. We speculate on the possible roles of phosphoinositides in membrane fusion and NE formation.