Nuclear envelope formation in vitro: A sea urchin egg cell-free system

Richard D. Byrne, Vanessa Zhendre, Banafshé Larijani, Dominic L. Poccia

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The formation of the nuclear envelope (NE) typically occurs once during every mitotic cycle in somatic cells, and also around the sperm nucleus following fertilization. Much of our understanding of NE assembly has been derived from systems modeling the latter event in vitro. In these systems, demembranated sperm nuclei are combined with fertilized egg cytoplasmic extracts and an ATP-regenerating system and in a multistep process they form the functional double bilayer of the NE. Using a system that we developed from sea urchin gametes, we have demonstrated that NE assembly is regulated by membrane vesicles in a spatial and temporal fashion, emphasizing the roles of phosphoinositides, particularly phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), diacylglycerols (DAG), and lipid-modifying enzymes in NE assembly.

Original languageEnglish
Title of host publicationThe Nucleus
Subtitle of host publicationVolume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging
PublisherHumana Press
Pages207-223
Number of pages17
ISBN (Print)9781603274609
DOIs
Publication statusPublished - 1 Jan 2008

Publication series

NameMethods in Molecular Biology
Volume464
ISSN (Print)1064-3745

Keywords

  • Cholesterol
  • Diacylglycerol
  • Liquid NMR
  • Liquid nuclear magnetic resonance
  • Nuclear envelope
  • Phosphatidylcholine
  • Phosphatidylinositol
  • Phospholipase C
  • PtdIns(4;5)P2
  • Sea urchin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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