Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL

Maria K. Vartiainen, Sebastian Guettler, Banafshe Larijani, Richard Treisman

Research output: Contribution to journalArticlepeer-review

Abstract

Actin, which is best known as a cytoskeletal component, also participates in the control of gene expression. We report a function of nuclear actin in the regulation of MAL, a coactivator of the transcription factor serum response factor (SRF). MAL, which binds monomelic actin, is cytoplasmic in many cells but accumulates in the nucleus upon serum-induced actin polymerization. MAL rapidly shuttles between cytoplasm and nucleus in unstimulated cells. Serum stimulation effectively blocks MAL nuclear export, which requires MAL-actin interaction. Nuclear MAL binds SRF target genes but remains inactive unless actin binding is disrupted. Fluorescence resonance energy transfer analysis demonstrates that the MAL-actin interaction responds to extracellular signals. Serum-induced signaling is thus communicated to nuclear actin to control a transcriptional regulator.

Original languageEnglish
Pages (from-to)1749-1752
Number of pages4
JournalScience
Volume316
Issue number5832
DOIs
Publication statusPublished - 22 Jun 2007

ASJC Scopus subject areas

  • General

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