Nozzle-free electrospinning of Polyvinylpyrrolidone/Poly(glycerol sebacate) fibrous scaffolds for skin tissue engineering applications

Antonios Keirouz; Giuseppino Fortunato; Mei Zhang; Anthony Callanan; Norbert Radacsi

doi:10.1016/j.medengphy.2019.06.009

Nozzle-free electrospinning of Polyvinylpyrrolidone/Poly(glycerol sebacate) fibrous scaffolds for skin tissue engineering applications

Antonios Keirouz, Giuseppino Fortunato, Mei Zhang, Anthony Callanan, Norbert Radacsi

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

41 Citations (SciVal)

Abstract

A novel composite for skin tissue engineering applications by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly (glycerol sebacate) (PGS) was fabricated via the scalable nozzle-free electrospinning technique. The formed PVP:PGS blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers correlated to the blend ratio. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of the composite fibers significantly decreased the construct's wettability and stabilized the formed fiber mats, which was indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblast cells. The present findings provide valuable insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer as a promising future candidate for skin substitute constructs.

Original language	English
Pages (from-to)	56-67
Number of pages	12
Journal	Medical Engineering and Physics
Volume	71
Early online date	27 Jun 2019
DOIs	https://doi.org/10.1016/j.medengphy.2019.06.009
Publication status	Published - 1 Sept 2019

Bibliographical note

Funding Information:
We would like to thank Dr. Junxi Wu from the Centre of Cardiovascular Science of The University of Edinburgh for donating the cell-line used for the viability testing. We thank Professor Vasileios Koutsos and Dr. Colin Robert of The University of Edinburgh for their valued suggestions. The authors also thank Dr. Coinneach Dover and Dr. Mike Davidson of The University of Edinburgh for providing access and training on the water contact angle measurement and FTIR equipment, respectively. We would also like to thank Fergus Dingwall, Mariia Zakharova and Jae Hoon Kwon of The University of Edinburgh for their appreciated laboratory assistance.

Publisher Copyright:
© 2019 IPEM

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

Elastic
Nozzle-free electrospinning
PGS
Polymer
PVP
UV

ASJC Scopus subject areas

Biophysics
Biomedical Engineering

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10.1016/j.medengphy.2019.06.009

Cite this

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title = "Nozzle-free electrospinning of Polyvinylpyrrolidone/Poly(glycerol sebacate) fibrous scaffolds for skin tissue engineering applications",

abstract = "A novel composite for skin tissue engineering applications by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly (glycerol sebacate) (PGS) was fabricated via the scalable nozzle-free electrospinning technique. The formed PVP:PGS blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers correlated to the blend ratio. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of the composite fibers significantly decreased the construct's wettability and stabilized the formed fiber mats, which was indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblast cells. The present findings provide valuable insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer as a promising future candidate for skin substitute constructs.",

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author = "Antonios Keirouz and Giuseppino Fortunato and Mei Zhang and Anthony Callanan and Norbert Radacsi",

note = "Funding Information: We would like to thank Dr. Junxi Wu from the Centre of Cardiovascular Science of The University of Edinburgh for donating the cell-line used for the viability testing. We thank Professor Vasileios Koutsos and Dr. Colin Robert of The University of Edinburgh for their valued suggestions. The authors also thank Dr. Coinneach Dover and Dr. Mike Davidson of The University of Edinburgh for providing access and training on the water contact angle measurement and FTIR equipment, respectively. We would also like to thank Fergus Dingwall, Mariia Zakharova and Jae Hoon Kwon of The University of Edinburgh for their appreciated laboratory assistance. Publisher Copyright: {\textcopyright} 2019 IPEM Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

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AU - Callanan, Anthony

AU - Radacsi, Norbert

N1 - Funding Information: We would like to thank Dr. Junxi Wu from the Centre of Cardiovascular Science of The University of Edinburgh for donating the cell-line used for the viability testing. We thank Professor Vasileios Koutsos and Dr. Colin Robert of The University of Edinburgh for their valued suggestions. The authors also thank Dr. Coinneach Dover and Dr. Mike Davidson of The University of Edinburgh for providing access and training on the water contact angle measurement and FTIR equipment, respectively. We would also like to thank Fergus Dingwall, Mariia Zakharova and Jae Hoon Kwon of The University of Edinburgh for their appreciated laboratory assistance. Publisher Copyright: © 2019 IPEM Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

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N2 - A novel composite for skin tissue engineering applications by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly (glycerol sebacate) (PGS) was fabricated via the scalable nozzle-free electrospinning technique. The formed PVP:PGS blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers correlated to the blend ratio. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of the composite fibers significantly decreased the construct's wettability and stabilized the formed fiber mats, which was indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblast cells. The present findings provide valuable insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer as a promising future candidate for skin substitute constructs.

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Nozzle-free electrospinning of Polyvinylpyrrolidone/Poly(glycerol sebacate) fibrous scaffolds for skin tissue engineering applications

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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