Abstract
Advances in materials science have accelerated the development of diagnostic tools with the last decade witnessing the development of enzyme-free sensors, owing to the improved stability, low cost and simple fabrication of component materials. However, the specificity of non-enzymatic sensors for certain analytes still represents a challenging task, for example the determination of cholesterol level in blood is vital due to its medical relevance. In this work, a reagent displacement assay for cholesterol sensing in serum samples was developed. It is based on coating of a glassy carbon electrode with a polymer of intrinsic microporosity (PIM) that forms a host-guest complex with methylene blue (MB). In the presence of cholesterol, the MB electroactive probe was displaced due to the stronger association of cholesterol guest to the PIM host. The decrease in the oxidative current was proportional to the cholesterol concentration achieving a detection limit of approximately 0.1 nM. Moreover, to further assist the experimental studies, comprehensive theoretical calculations are also performed by using density functional theory (DFT) calculations. Graphical abstract: [Figure not available: see fulltext.].
Original language | English |
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Article number | 72 |
Pages (from-to) | 6523-6533 |
Number of pages | 11 |
Journal | Analytical and Bioanalytical Chemistry |
Volume | 413 |
Issue number | 26 |
Early online date | 31 Aug 2021 |
DOIs | |
Publication status | Published - 30 Nov 2021 |
Bibliographical note
Funding Information:The authors received support of this work from the University of Mohaghegh Ardabili Research Council, Ardabil, Iran (grant number 3051234502).
Keywords
- Cholesterol
- DFT calculations
- Electrochemical sensor
- Methylene blue
- Polymer of intrinsic microporosity (PIM)
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry