Nine months of combined training improves ex vivo skeletal muscle metabolism in individuals with type 2 diabetes

L.M. Sparks, N.M. Johannsen, T.S. Church, C.P. Earnest, E. Moonen-Kornips, C. Moro, M.K.C. Hesselink, S.R. Smith, P. Schrauwen

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Context: Type 2 diabetes (T2D) has features of disordered lipid and glucose metabolism, due in part to reduced mitochondrial content. Objective: Our objective was to investigate effects of different types of exercise on mitochondrial content and substrate oxidation in individuals with T2D (ancillary study of the randomized controlled trial Health Benefits of Aerobic and Resistance Training in Individuals with Type 2 Diabetes, HART-D). Intervention: T2D individuals were randomized to aerobictraining (AT, n = 12), resistance training (RT, n = 18), combination training (ATRT, n = 12), or nonexercise control (n = 10). Blood draws, peak oxygen consumption tests, dual-energy x-ray absorptiometry scans and muscle biopsies of vastus lateralis were performed before and after 9 months. Ex vivo substrate oxidations (14CO2), mitochondrial content, and enzyme activities were measured. Glycated hemoglobin A and free fatty acids were also determined. Results: Mitochondrial content increased after RT and ATRT. Octanoate oxidation increased after AT and ATRT, whereas palmitate, pyruvate, and acetate oxidations increased in all exercise groups. Exercise-induced responses in mitochondrial DNA were associated with improvements in peak oxygen consumption, β-hydroxyacyl-coenzyme A dehydrogenase activity, and palmitate oxidation. Conclusions: Nine months of AT and RT significantly improved most aspects of skeletal muscle mitochondrial content and substrate oxidation, whereas the combination improved all aspects. These exercise responses were associated with clinical improvements, indicating that long-term training, especially combination, is an effective lifestyle therapy for individuals with T2D by way of improving muscle substrate metabolism.

Original languageEnglish
Pages (from-to)1694-1702
Number of pages9
JournalJournal of Clinical Endocrinology & Metabolism
Volume98
Issue number4
Early online date5 Mar 2013
DOIs
Publication statusPublished - 1 Apr 2013

Fingerprint Dive into the research topics of 'Nine months of combined training improves ex vivo skeletal muscle metabolism in individuals with type 2 diabetes'. Together they form a unique fingerprint.

  • Cite this

    Sparks, L. M., Johannsen, N. M., Church, T. S., Earnest, C. P., Moonen-Kornips, E., Moro, C., Hesselink, M. K. C., Smith, S. R., & Schrauwen, P. (2013). Nine months of combined training improves ex vivo skeletal muscle metabolism in individuals with type 2 diabetes. Journal of Clinical Endocrinology & Metabolism, 98(4), 1694-1702. https://doi.org/10.1210/jc.2012-3874