Neutron diffraction studies of viral fusion peptides

Jeremy P. Bradshaw, Malcolm J.m. Darkes, John Katsaras, Richard M. Epand

Research output: Contribution to journalConference article

12 Citations (Scopus)

Abstract

Membrane fusion plays a vital role in a large and diverse number of essential biological processes. Despite this fact, the precise molecular events that occur during fusion are still not known. We are currently engaged on a study of membrane fusion as mediated by viral fusion peptides. These peptides are the N-terminal regions of certain viral envelope proteins that mediate the process of fusion between the viral envelope and the membranes of the host cell during the infection process. As part of this study, we have carried out neutron diffraction measurements at the ILL, BeNSC and Chalk River, on a range of viral fusion peptides. The peptides, from simian immunodeficiency virus (SIV), influenza A and feline leukaemia virus (FeLV), were incorporated into stacked phospholipid bilayers. Some of the peptides had been specifically deuterated at key amino acids. Lamellar diffraction data were collected and analyzed to yield information on the peptide conformation, location and orientation relative to the bilayer.

Original languageEnglish
Pages (from-to)495-498
Number of pages4
JournalPhysica B: Condensed Matter
Volume276-278
DOIs
Publication statusPublished - 1 Jan 2000
Event2nd European Conference on Neutron Scattering (ECNS '99) - Budapest, Hung
Duration: 1 Sep 19994 Sep 1999

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Neutron diffraction studies of viral fusion peptides. / Bradshaw, Jeremy P.; J.m. Darkes, Malcolm; Katsaras, John; Epand, Richard M.

In: Physica B: Condensed Matter, Vol. 276-278, 01.01.2000, p. 495-498.

Research output: Contribution to journalConference article

Bradshaw, Jeremy P. ; J.m. Darkes, Malcolm ; Katsaras, John ; Epand, Richard M. / Neutron diffraction studies of viral fusion peptides. In: Physica B: Condensed Matter. 2000 ; Vol. 276-278. pp. 495-498.
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