Neutron diffraction reveals the site of amantadine blockade in the influenza A M2 ion channel

K. C. Duff, P. J. Gilchrist, A. M. Saxena, J. P. Bradshaw

Research output: Contribution to journalArticle

Abstract

The influenza A M2 protein forms proton channels which are blocked by the anti-influenza drug amantadine. Using the technique of neutron diffraction with both deuterium-labeled amantadine and influenza A M2 peptides, this study has directly located the position of interaction between the drug and the transmembrane domain of M2. Amantadine is found 0.5 nm from the center of the bilayer in an area between Val 27 and Ser 31, a location consistent with the formation of a steric block within the ion channel. Similar experiments with amantadine and an amantadine-resistant mutant peptide showed no such interaction.

Original languageEnglish
Article number71345
Pages (from-to)287-293
Number of pages7
JournalVirology
Volume202
Issue number1
DOIs
Publication statusPublished - 1 Jan 1994

ASJC Scopus subject areas

  • Virology

Cite this

Neutron diffraction reveals the site of amantadine blockade in the influenza A M2 ion channel. / Duff, K. C.; Gilchrist, P. J.; Saxena, A. M.; Bradshaw, J. P.

In: Virology, Vol. 202, No. 1, 71345, 01.01.1994, p. 287-293.

Research output: Contribution to journalArticle

Duff, K. C. ; Gilchrist, P. J. ; Saxena, A. M. ; Bradshaw, J. P. / Neutron diffraction reveals the site of amantadine blockade in the influenza A M2 ion channel. In: Virology. 1994 ; Vol. 202, No. 1. pp. 287-293.
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