Neuronal cell biocompatibility and adhesion to modified CMOS electrodes

A H D Graham, C R Bowen, John Taylor, J Robbins

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The use of CMOS (Complementary Metal Oxide Semiconductor) integrated circuits to create electrodes for biosensors, implants and drug-discovery has several potential advantages over passive multi-electrode arrays (MEAs). However, unmodified aluminium CMOS electrodes may corrode in a physiological environment. We have investigated a low-cost electrode design based on the modification of CMOS metallisation to produce a nanoporous alumina electrode as an interface to mammalian neuronal cells and corrosion inhibitor. Using NG108-15 mouse neuroblastoma x rat glioma hybrid cells, results show that porous alumina is biocompatible and that the inter-pore distance (pore pitch) of the alumina has no effect on cell vitality. To establish whether porous alumina and a cell membrane can produce a tight junction required for good electrical coupling between electrode and cell, we devised a novel cell detachment centrifugation assay to assess the long-term adhesion of cells. Results show that porous alumina substrates produced with a large pore pitch of 206 nm present a significantly improved surface compared to the unmodified aluminium control and that small pore-pitches of 17 nm and 69 nm present a less favourable surface for cell adhesion.
Original languageEnglish
Pages (from-to)1091-1101
Number of pages11
JournalBiomedical Microdevices
Volume11
Issue number5
Early online date20 May 2009
DOIs
Publication statusPublished - 1 Oct 2009

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Semiconductors
Biocompatibility
Cell Adhesion
Aluminum Oxide
Oxides
Electrodes
Adhesion
Metals
Alumina
Aluminum
CMOS integrated circuits
Corrosion
Centrifugation
Hybrid Cells
Tight Junctions
Cell adhesion
Biosensing Techniques
Corrosion inhibitors
Drug Discovery
Cell membranes

Cite this

Neuronal cell biocompatibility and adhesion to modified CMOS electrodes. / Graham, A H D; Bowen, C R; Taylor, John; Robbins, J.

In: Biomedical Microdevices, Vol. 11, No. 5, 01.10.2009, p. 1091-1101.

Research output: Contribution to journalArticle

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