Neural Crest Methodologies in Zebrafish and Medaka

Kleio Petratou, Karen Camargo Sosa, Ruqaiya Al Jabri, Yusuke Nagao, Robert N. Kelsh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Neural crest cells are highly multipotent and strongly migratory cells and generate adult neural crest stem cells with varied roles in cellular homeostasis and regeneration. The optical transparency and ready accessibility of fish embryos make them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study, requiring that they be identified using marker expression. We describe key protocols for the analysis of neural crest marker expression in zebrafish and medaka, including whole-mount in situ hybridization to detect mRNA using conventional chromogenic substrates and the more recent RNAscope which gives readily multiplexed fluorescent detection and immunofluorescent detection of antigens.
Original languageEnglish
Title of host publicationMethods relevant to Neural Crest Research
EditorsQuentin Schwartz, Sophie Wizniak
PublisherSpringer
Chapter13
Pages167-184
Number of pages18
Volume1976
ISBN (Print)9781493994113
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1976

Keywords

  • Immunofluorescence
  • In situ hybridization
  • Medaka
  • Neural crest
  • Pigment cells
  • Protein detection RNAscope
  • Transcript detection
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Projects

JSPS Fellowship for Y.Nagao

Kelsh, R.

1/04/1731/03/19

Project: Other

Cite this

Petratou, K., Camargo Sosa, K., Al Jabri, R., Nagao, Y., & Kelsh, R. N. (2019). Neural Crest Methodologies in Zebrafish and Medaka. In Q. Schwartz, & S. Wizniak (Eds.), Methods relevant to Neural Crest Research (Vol. 1976, pp. 167-184). (Methods in Molecular Biology; Vol. 1976). Springer. https://doi.org/10.1007/978-1-4939-9412-0_13