Neural Crest Methodologies in Zebrafish and Medaka: Transcript and protein detection methodologies for neural crest research on whole mount zebrafish and medaka

Kleio Petratou, Karen Camargo Sosa, Ruqaiya Al Jabri, Yusuke Nagao, Robert N. Kelsh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Neural crest cells are highly multipotent and strongly migratory cells and generate adult neural crest stem cells with varied roles in cellular homeostasis and regeneration. The optical transparency and ready accessibility of fish embryos make them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study, requiring that they be identified using marker expression. We describe key protocols for the analysis of neural crest marker expression in zebrafish and medaka, including whole-mount in situ hybridization to detect mRNA using conventional chromogenic substrates and the more recent RNAscope which gives readily multiplexed fluorescent detection and immunofluorescent detection of antigens.
Original languageEnglish
Title of host publicationMethods relevant to Neural Crest Research
EditorsQuentin Schwartz, Sophie Wizniak
PublisherSpringer
ISBN (Print)9781493994113
DOIs
Publication statusE-pub ahead of print - 12 Apr 2019

Publication series

NameMethods in Molecular Biology
Volume1976

Cite this

Petratou, K., Camargo Sosa, K., Al Jabri, R., Nagao, Y., & Kelsh, R. N. (2019). Neural Crest Methodologies in Zebrafish and Medaka: Transcript and protein detection methodologies for neural crest research on whole mount zebrafish and medaka. In Q. Schwartz, & S. Wizniak (Eds.), Methods relevant to Neural Crest Research (Methods in Molecular Biology; Vol. 1976). Springer. https://doi.org/10.1007/978-1-4939-9412-0_13

Neural Crest Methodologies in Zebrafish and Medaka: Transcript and protein detection methodologies for neural crest research on whole mount zebrafish and medaka. / Petratou, Kleio; Camargo Sosa, Karen; Al Jabri, Ruqaiya; Nagao, Yusuke; Kelsh, Robert N.

Methods relevant to Neural Crest Research. ed. / Quentin Schwartz; Sophie Wizniak. Springer, 2019. (Methods in Molecular Biology; Vol. 1976).

Research output: Chapter in Book/Report/Conference proceedingChapter

Petratou, K, Camargo Sosa, K, Al Jabri, R, Nagao, Y & Kelsh, RN 2019, Neural Crest Methodologies in Zebrafish and Medaka: Transcript and protein detection methodologies for neural crest research on whole mount zebrafish and medaka. in Q Schwartz & S Wizniak (eds), Methods relevant to Neural Crest Research. Methods in Molecular Biology, vol. 1976, Springer. https://doi.org/10.1007/978-1-4939-9412-0_13
Petratou K, Camargo Sosa K, Al Jabri R, Nagao Y, Kelsh RN. Neural Crest Methodologies in Zebrafish and Medaka: Transcript and protein detection methodologies for neural crest research on whole mount zebrafish and medaka. In Schwartz Q, Wizniak S, editors, Methods relevant to Neural Crest Research. Springer. 2019. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9412-0_13
Petratou, Kleio ; Camargo Sosa, Karen ; Al Jabri, Ruqaiya ; Nagao, Yusuke ; Kelsh, Robert N. / Neural Crest Methodologies in Zebrafish and Medaka: Transcript and protein detection methodologies for neural crest research on whole mount zebrafish and medaka. Methods relevant to Neural Crest Research. editor / Quentin Schwartz ; Sophie Wizniak. Springer, 2019. (Methods in Molecular Biology).
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