Near-infrared imaging for visualizing the synergistic relationship between autophagy and NFS1 protein during multidrug resistance using an ICT-TICT integrated platform

Wei Hu, Yifan He, Haixian Ren, Li Chai, Haiyan Li, Jianbin Chen, Chunya Li, Yanying Wang, Tony D. James

Research output: Contribution to journalArticlepeer-review


Drug resistance is a major challenge for cancer treatment, and its identification is crucial for medical research. However, since drug resistance is a multi-faceted phenomenon, it is important to simultaneously evaluate multiple target fluctuations. Recently developed fluorescence-based probes that can simultaneously respond to multiple targets offer many advantages for real-time and in situ monitoring of cellular metabolism, including ease of operation, rapid reporting, and their non-invasive nature. As such we developed a dual-response platform (Vis-H2S) with integrated ICT-TICT to image H2S and viscosity in mitochondria, which could simultaneously track fluctuations in cysteine desulfurase (NFS1 protein and H2S inducer) and autophagy during chemotherapy-induced multidrug resistance. This platform could monitor multiple endogenous metabolites and the synergistic relationship between autophagy and NFS1 protein during multidrug resistance induced by chemotherapy. The results indicated that chemotherapeutic drugs simultaneously up-regulate the levels of NFS1 protein and autophagy. It was also found that the NFS1 protein was linked with autophagy, which eventually led to multidrug resistance. As such, this platform could serve as an effective tool for the in-depth exploration of drug resistance mechanisms.

Original languageEnglish
Pages (from-to)6028-6035
Number of pages8
JournalChemical Science
Issue number16
Early online date25 Mar 2024
Publication statusPublished - 28 Apr 2024

Data Availability Statement

All data supporting this study are provided as ESI† accompanying this paper.

ASJC Scopus subject areas

  • General Chemistry

Cite this