Near-Infrared Fluorescent Probe for the In Situ Visualization of Oxidative Stress in the Brains of Neuroinflammatory and Schizophrenic Mice

Yujie Geng, Hanchen Zhang, Guoyang Zhang, Jiaying Zhou, Mingguang Zhu, Lijun Ma, Xuefei Wang, Tony D. James, Zhuo Wang

Research output: Contribution to journalArticlepeer-review

2 Citations (SciVal)

Abstract

Schizophrenia is a common mental disorder with unclear mechanisms. Oxidative stress and neuroinflammation play important roles in the pathological process of schizophrenia. Superoxide anion (O2•-) is an important oxidative stress biomarker in vivo. However, due to the existence of the blood-brain barrier (BBB), few near-infrared (NIR) fluorescent probes have been used for the sensing and detection of O2•- in the brain. With this research, we developed the first near-infrared fluorescent probe (named CT-CF3) for noninvasive detection of endogenous O2•- in the brain of mice. Enabling fluorescence monitoring of the dynamic changes in O2•- flux due to the prolonged activation of microglia in neuroinflamed and schizophrenic (SZ) mice brains, thereby providing direct evidence for the relationship between oxidative stress, neuroinflammation, and schizophrenia. Furthermore, we confirmed the O2•- burst in the brains of first-episode schizophrenic mice and assessed the effect of two atypical antipsychotic drugs (risperidone and olanzapine) on redox homeostasis.

Original languageEnglish
Pages (from-to)11943-11952
Number of pages10
JournalAnalytical Chemistry
Volume95
Issue number32
Early online date1 Aug 2023
DOIs
Publication statusPublished - 15 Aug 2023

Bibliographical note

Funding Information:
The authors are thankful for the support from the Beijing Natural Science Foundation (No. 7232342), the National Key Research and Development Program of China (2021YFC2101500) Academy of Medical Sciences Newton Advanced Fellowship (NAFR13\1015), and the China Scholarship Council. X.F.W. thanks the Beijing Natural Science Foundation (No. 8222074). T.D.J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). Animal studies were approved by the Ethical Committee China-Japan Friendship Hospital and performed under legal protocols. The approval number is zryhyy12-20-10-2.

Funding Information:
The authors are thankful for the support from the Beijing Natural Science Foundation (No. 7232342), the National Key Research and Development Program of China (2021YFC2101500) Academy of Medical Sciences Newton Advanced Fellowship (NAFR13\1015), and the China Scholarship Council. X.F.W. thanks the Beijing Natural Science Foundation (No. 8222074). T.D.J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). Animal studies were approved by the Ethical Committee China–Japan Friendship Hospital and performed under legal protocols. The approval number is zryhyy12-20-10-2.

ASJC Scopus subject areas

  • Analytical Chemistry

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