Near-Infrared Fluorescent Probe for the In Situ Visualization of Oxidative Stress in the Brains of Neuroinflammatory and Schizophrenic Mice

Yujie Geng; Hanchen Zhang; Guoyang Zhang; Jiaying Zhou; Mingguang Zhu; Lijun Ma; Xuefei Wang; Tony D. James; Zhuo Wang

doi:10.1021/acs.analchem.3c01447

Near-Infrared Fluorescent Probe for the In Situ Visualization of Oxidative Stress in the Brains of Neuroinflammatory and Schizophrenic Mice

Yujie Geng, Hanchen Zhang, Guoyang Zhang, Jiaying Zhou, Mingguang Zhu, Lijun Ma, Xuefei Wang, Tony D. James, Zhuo Wang

Research output: Contribution to journal › Article › peer-review

10 Citations (SciVal)

Abstract

Schizophrenia is a common mental disorder with unclear mechanisms. Oxidative stress and neuroinflammation play important roles in the pathological process of schizophrenia. Superoxide anion (O₂^•-) is an important oxidative stress biomarker in vivo. However, due to the existence of the blood-brain barrier (BBB), few near-infrared (NIR) fluorescent probes have been used for the sensing and detection of O₂^•- in the brain. With this research, we developed the first near-infrared fluorescent probe (named CT-CF₃) for noninvasive detection of endogenous O₂^•- in the brain of mice. Enabling fluorescence monitoring of the dynamic changes in O₂^•- flux due to the prolonged activation of microglia in neuroinflamed and schizophrenic (SZ) mice brains, thereby providing direct evidence for the relationship between oxidative stress, neuroinflammation, and schizophrenia. Furthermore, we confirmed the O₂^•- burst in the brains of first-episode schizophrenic mice and assessed the effect of two atypical antipsychotic drugs (risperidone and olanzapine) on redox homeostasis.

Original language	English
Pages (from-to)	11943-11952
Number of pages	10
Journal	Analytical Chemistry
Volume	95
Issue number	32
Early online date	1 Aug 2023
DOIs	https://doi.org/10.1021/acs.analchem.3c01447
Publication status	Published - 15 Aug 2023

Bibliographical note

Funding Information:
The authors are thankful for the support from the Beijing Natural Science Foundation (No. 7232342), the National Key Research and Development Program of China (2021YFC2101500) Academy of Medical Sciences Newton Advanced Fellowship (NAFR13\1015), and the China Scholarship Council. X.F.W. thanks the Beijing Natural Science Foundation (No. 8222074). T.D.J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). Animal studies were approved by the Ethical Committee China-Japan Friendship Hospital and performed under legal protocols. The approval number is zryhyy12-20-10-2.

Funding Information:
The authors are thankful for the support from the Beijing Natural Science Foundation (No. 7232342), the National Key Research and Development Program of China (2021YFC2101500) Academy of Medical Sciences Newton Advanced Fellowship (NAFR13\1015), and the China Scholarship Council. X.F.W. thanks the Beijing Natural Science Foundation (No. 8222074). T.D.J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). Animal studies were approved by the Ethical Committee China–Japan Friendship Hospital and performed under legal protocols. The approval number is zryhyy12-20-10-2.

ASJC Scopus subject areas

Analytical Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

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title = "Near-Infrared Fluorescent Probe for the In Situ Visualization of Oxidative Stress in the Brains of Neuroinflammatory and Schizophrenic Mice",

abstract = "Schizophrenia is a common mental disorder with unclear mechanisms. Oxidative stress and neuroinflammation play important roles in the pathological process of schizophrenia. Superoxide anion (O2•-) is an important oxidative stress biomarker in vivo. However, due to the existence of the blood-brain barrier (BBB), few near-infrared (NIR) fluorescent probes have been used for the sensing and detection of O2•- in the brain. With this research, we developed the first near-infrared fluorescent probe (named CT-CF3) for noninvasive detection of endogenous O2•- in the brain of mice. Enabling fluorescence monitoring of the dynamic changes in O2•- flux due to the prolonged activation of microglia in neuroinflamed and schizophrenic (SZ) mice brains, thereby providing direct evidence for the relationship between oxidative stress, neuroinflammation, and schizophrenia. Furthermore, we confirmed the O2•- burst in the brains of first-episode schizophrenic mice and assessed the effect of two atypical antipsychotic drugs (risperidone and olanzapine) on redox homeostasis.",

author = "Yujie Geng and Hanchen Zhang and Guoyang Zhang and Jiaying Zhou and Mingguang Zhu and Lijun Ma and Xuefei Wang and James, {Tony D.} and Zhuo Wang",

note = "Funding Information: The authors are thankful for the support from the Beijing Natural Science Foundation (No. 7232342), the National Key Research and Development Program of China (2021YFC2101500) Academy of Medical Sciences Newton Advanced Fellowship (NAFR13\1015), and the China Scholarship Council. X.F.W. thanks the Beijing Natural Science Foundation (No. 8222074). T.D.J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). Animal studies were approved by the Ethical Committee China-Japan Friendship Hospital and performed under legal protocols. The approval number is zryhyy12-20-10-2. Funding Information: The authors are thankful for the support from the Beijing Natural Science Foundation (No. 7232342), the National Key Research and Development Program of China (2021YFC2101500) Academy of Medical Sciences Newton Advanced Fellowship (NAFR13\1015), and the China Scholarship Council. X.F.W. thanks the Beijing Natural Science Foundation (No. 8222074). T.D.J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). Animal studies were approved by the Ethical Committee China–Japan Friendship Hospital and performed under legal protocols. The approval number is zryhyy12-20-10-2. ",

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doi = "10.1021/acs.analchem.3c01447",

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AU - Geng, Yujie

AU - Zhang, Hanchen

AU - Zhang, Guoyang

AU - Zhou, Jiaying

AU - Zhu, Mingguang

AU - Ma, Lijun

AU - Wang, Xuefei

AU - James, Tony D.

AU - Wang, Zhuo

N1 - Funding Information: The authors are thankful for the support from the Beijing Natural Science Foundation (No. 7232342), the National Key Research and Development Program of China (2021YFC2101500) Academy of Medical Sciences Newton Advanced Fellowship (NAFR13\1015), and the China Scholarship Council. X.F.W. thanks the Beijing Natural Science Foundation (No. 8222074). T.D.J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). Animal studies were approved by the Ethical Committee China-Japan Friendship Hospital and performed under legal protocols. The approval number is zryhyy12-20-10-2. Funding Information: The authors are thankful for the support from the Beijing Natural Science Foundation (No. 7232342), the National Key Research and Development Program of China (2021YFC2101500) Academy of Medical Sciences Newton Advanced Fellowship (NAFR13\1015), and the China Scholarship Council. X.F.W. thanks the Beijing Natural Science Foundation (No. 8222074). T.D.J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). Animal studies were approved by the Ethical Committee China–Japan Friendship Hospital and performed under legal protocols. The approval number is zryhyy12-20-10-2.

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N2 - Schizophrenia is a common mental disorder with unclear mechanisms. Oxidative stress and neuroinflammation play important roles in the pathological process of schizophrenia. Superoxide anion (O2•-) is an important oxidative stress biomarker in vivo. However, due to the existence of the blood-brain barrier (BBB), few near-infrared (NIR) fluorescent probes have been used for the sensing and detection of O2•- in the brain. With this research, we developed the first near-infrared fluorescent probe (named CT-CF3) for noninvasive detection of endogenous O2•- in the brain of mice. Enabling fluorescence monitoring of the dynamic changes in O2•- flux due to the prolonged activation of microglia in neuroinflamed and schizophrenic (SZ) mice brains, thereby providing direct evidence for the relationship between oxidative stress, neuroinflammation, and schizophrenia. Furthermore, we confirmed the O2•- burst in the brains of first-episode schizophrenic mice and assessed the effect of two atypical antipsychotic drugs (risperidone and olanzapine) on redox homeostasis.

AB - Schizophrenia is a common mental disorder with unclear mechanisms. Oxidative stress and neuroinflammation play important roles in the pathological process of schizophrenia. Superoxide anion (O2•-) is an important oxidative stress biomarker in vivo. However, due to the existence of the blood-brain barrier (BBB), few near-infrared (NIR) fluorescent probes have been used for the sensing and detection of O2•- in the brain. With this research, we developed the first near-infrared fluorescent probe (named CT-CF3) for noninvasive detection of endogenous O2•- in the brain of mice. Enabling fluorescence monitoring of the dynamic changes in O2•- flux due to the prolonged activation of microglia in neuroinflamed and schizophrenic (SZ) mice brains, thereby providing direct evidence for the relationship between oxidative stress, neuroinflammation, and schizophrenia. Furthermore, we confirmed the O2•- burst in the brains of first-episode schizophrenic mice and assessed the effect of two atypical antipsychotic drugs (risperidone and olanzapine) on redox homeostasis.

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JO - Analytical Chemistry

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ER -

Near-Infrared Fluorescent Probe for the In Situ Visualization of Oxidative Stress in the Brains of Neuroinflammatory and Schizophrenic Mice

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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