Near-infrared fluorescent probe for hydrogen sulfide: High-fidelity ferroptosis evaluation in vivo during stroke

Tianyu Liang, Taotao Qiang, Longfang Ren, Fei Cheng, Baoshuai Wang, Mingli Li, Wei Hu, Tony D. James

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53 Citations (SciVal)


Ferroptosis is closely associated with cancer, neurodegenerative diseases and ischemia-reperfusion injury and the detection of its pathological process is very important for early disease diagnosis. Fluorescence based sensing technologies have become excellent tools due to the real-time detection of cellular physiological or pathological processes. However, to date the detection of ferroptosis using reducing substances as markers has not been achieved since the reducing substances are not only present at extremely low concentrations during ferroptosis but also play a key role in the further development of ferroptosis. Significantly, sensors for reducing substances usually consume reducing substances, instigating a redox imbalance, which further aggravates the progression of ferroptosis. In this work, a H2S triggered and H2S releasing near-infrared fluorescent probe (HL-H2S) was developed for the high-fidelity in situ imaging of ferroptosis. In the imaging process, HL-H2S consumes H2S and releases carbonyl sulfide, which is then catalyzed by carbonic anhydrase to produce H2S. Importantly, this strategy does not intensify ferroptosis since it avoids disruption of the redox homeostasis. Furthermore, using erastin as an inducer for ferroptosis, the observed trends for Fe2+, MDA, and GSH, indicate that the introduction of the HL-H2S probe does not exacerbate ferroptosis. In contrast, ferroptosis progression was significantly promoted when the release of H2S from HL-H2S was inhibited using AZ. These results indicate that the H2S triggered and H2S releasing fluorescent probe did not interfere with the progression of ferroptosis, thus enabling high-fidelity in situ imaging of ferroptosis.

Original languageEnglish
Pages (from-to)2992-3001
Number of pages10
JournalChemical Science
Issue number10
Early online date21 Feb 2022
Publication statusPublished - 28 Apr 2022

Bibliographical note

Funding Information:
All animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of South-Central University of Nationalities and experiments were approved by the Animal Ethics Committee of College of Biology (South-Central University of Nationalities). This work was funded by the Open Project of Shaanxi Collaborative Innovation Center of Industrial Auxiliary Chemistry & Technology (No. XTKF-2020-05); the Key Scientific Research Group of Shaanxi Province (2020TD-009), Key Scientific Research Program of Shaanxi Provincial Education Department (Collaborative Innovation Center project) (20JY003), Science and Technology Plan Project of Xi'an Weiyang District (No. 201907) and the Youth Innovation Team of Shaanxi Universities. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01).

ASJC Scopus subject areas

  • General Chemistry


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