Native RNA-Sequencing Throws its Hat into the Transcriptomics Ring

Shobbir Hussain

doi:10.1016/j.tibs.2018.02.007

Native RNA-Sequencing Throws its Hat into the Transcriptomics Ring

Shobbir Hussain

Department of Life Sciences

Research output: Contribution to journal › Review article › peer-review

14 Citations (SciVal)

127 Downloads (Pure)

Abstract

De novo sequence-level surveys of transcriptomes have previously relied on sequencing via a DNA intermediate. While such methods can yield massive data sets, various problems mean that these do not always accurately reflect the true innate composition of transcriptomes. Enter Garalde et al., who present for the first time highly parallel native RNA-Sequencing (RNA-seq), with potentially disruptive future-implications for the transcriptomics field.

Original language	English
Pages (from-to)	225-227
Number of pages	3
Journal	Trends in Biochemical Sciences
Volume	43
Issue number	4
Early online date	1 Mar 2018
DOIs	https://doi.org/10.1016/j.tibs.2018.02.007
Publication status	Published - 1 Apr 2018

Keywords

MinION
ONT
Oxford Nanopore Technologies
RNA-Seq
alternative splicing
epitranscriptome
epitranscriptomics
nanopore sequencing

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1016/j.tibs.2018.02.007

NatRNASeq_Manuscript_SH_Accepted (PDF)
The final published version is available via: https://doi.org/10.1016/j.tibs.2018.02.007
Accepted author manuscript, 812 KB

Cite this

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abstract = "De novo sequence-level surveys of transcriptomes have previously relied on sequencing via a DNA intermediate. While such methods can yield massive data sets, various problems mean that these do not always accurately reflect the true innate composition of transcriptomes. Enter Garalde et al., who present for the first time highly parallel native RNA-Sequencing (RNA-seq), with potentially disruptive future-implications for the transcriptomics field.",

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KW - MinION

KW - ONT

KW - Oxford Nanopore Technologies

KW - RNA-Seq

KW - alternative splicing

KW - epitranscriptome

KW - epitranscriptomics

KW - nanopore sequencing

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JO - Trends in Biochemical Sciences

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Native RNA-Sequencing Throws its Hat into the Transcriptomics Ring

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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Long-read Nanopore Sequencing to Identify Isoform-Specific Patterns of mRNA Methylation

Cite this

Native RNA-Sequencing Throws its Hat into the Transcriptomics Ring

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Long-read Nanopore Sequencing to Identify Isoform-Specific Patterns of mRNA Methylation

Cite this