NAD-sensitive hydrogel for the release of macromolecules

M Tang, R Zhang, Adrian Bowyer, R Eisenthal, J Hubble

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17 Citations (SciVal)


A hydrogel membrane contg. immobilized ligands and receptors was synthesized and investigated for the controlled diffusion of test proteins (cytochrome C and Hb). Both Cibacron blue (ligand) and lysozyme (receptor) were covalently linked to dextran mols. that were subsequently crosslinked to form a gel. The resulting stable hydrogels contained both covalent and affinity cross-links such that their intrinsic porosities were sensitive to competitive displacers of the affinity interaction between lysozyme and Cibacron blue. Transport expts. in a twin chamber diffusion cell showed that as NAD was added to the donor side, the dissocn. of the binding sites between the Cibacron blue and the lysozyme led to an increase in protein diffusion through the hydrogel. The results showed that addn. of NAD caused a saturable concn.-dependent increase in the transport of both cytochrome C and Hb. This effect was shown to be both specific and reversible.
Original languageEnglish
Pages (from-to)791-796
Number of pages6
JournalBiotechnology and Bioengineering
Issue number6
Publication statusPublished - 2004


  • NAD-sensitive hydrogel for release of macromols.)
  • dextran Cibacron blue lysozyme hydrogel macromol
  • BSU (Biological study
  • Drug delivery systems (hydrogels
  • Biological transport (diffusion
  • Dissolution
  • unclassified)
  • Hemoglobins Role
  • Porosity (NAD-sensitive hydrogel for release of macromols.)
  • BIOL (Biological study) (NAD-sensitive hydrogel for release of macromols.)


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